Immunoreactivity of specific epitopes of PrPSc is enhanced by pretreatment in a hydrated autoclave.

机译：通过在水合高压釜中进行预处理可以增强PrPSc特定表位的免疫反应性。

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An abnormal protein (PrPSc) accumulates in animals affected with scrapie. Immunoblotting procedures have been used widely to detect PrPSc. Blotted membranes were subjected to pretreatment in a hydrated autoclave, and the subsequent immunoreactivity of PrPSc was examined. The immunoreactivity of PrPSc to antisera against the synthetic peptides of the mouse PrP amino acid sequences 199 to 208 and 213 to 226 was enhanced by the pretreatment. However, the reactivity to antisera of peptide sequences 100 to 115 and 165 to 174 was not affected. The antibody-binding ability of the specific epitopes which are located close to the C-terminal end of PrP27-30 the proteinase-resistant portion of PrPSc, was enhanced by pretreatment in a hydrated autoclave. This pretreatment increased the sensitivity of PrPSc, and it would be useful for diagnosis of scrapie.

机译：异常蛋白（PrPSc）会在患有瘙痒病的动物体内积聚。免疫印迹程序已被广泛用于检测PrPSc。印迹的膜在水合高压釜中进行预处理，并检查随后的PrPSc免疫反应性。通过预处理增强了PrPSc针对抗血清的抗小鼠PrP氨基酸序列199至208和213至226的合成肽的免疫反应性。然而，肽序列100至115和165至174的抗血清的反应性不受影响。通过在水合高压釜中进行预处理，增强了靠近PrP27-30的C末端（即PrPSc的蛋白酶抗性部分）的C-末端的特定表位的抗体结合能力。这种预处理增加了PrPSc的敏感性，对诊断瘙痒病很有用。

著录项

期刊名称 Clinical and Diagnostic Laboratory Immunology
作者
T Yokoyama; E Momotani; K Kimura; N Yuasa;
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作者单位

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年(卷),期 1996(3),4
年度 1996
页码 470–471
总页数 2
原文格式 PDF
正文语种
中图分类免疫学;
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专利

1. Immunoreactivity of specific epitopes of PrPSc is enhanced by pretreatment in a hydrated autoclave. [J] . T Yokoyama, E Momotani, K Kimura, Clinical and diagnostic laboratory immunology . 1996,第4期

机译：通过在水合高压釜中进行预处理，可以增强PrPSc特定表位的免疫反应性。
2. Safety, specificity and immunogenicity of a PrPSc-specific prion vaccine based on the YYR disease specific epitope [J] . Prion . 2014,第1期

机译：基于YYR疾病特异性表位的PrPSc特异性病毒疫苗的安全性，特异性和免疫原性
3. Immunoreactivity enhancement with chelators for increasing the detection sensitivity of human PrPSc by Western blotting. [J] . Kuczius T, Brinkmann B, Grassi J Neurochemistry International: The International Journal for the Rapid Publication of Critical Reviews, Preliminary and Original Research Communications in Neurochemistry . 2007,第1期

机译：用螯合剂增强免疫反应性，以通过蛋白质印迹法提高人PrPSc的检测灵敏度。
4. Substitution of certain amino acids in a short peptide causes a significant difference in their immunoreactivities with antibodies against different epitopes: evidence for possible folding of the peptide on nitrocellulose or PVDF membrane [C] . Matsuo Taisuke, Yamamoto Takenori, Katsuda Chie, International Symposium on Micro-NanoMechatronics and Human Science . 2008

机译：在短肽中取代某些氨基酸导致其与针对不同表位的免疫反应的显着差异：肽在硝化纤维素或PVDF膜上可能折叠的证据
5. B cell epitopes of an Ehrlichia canis major immunoreactive glycoprotein (gp200) are defined by carbohydrate and peptide determinants [D] . Nethery, Kimberly A. 2006

机译：犬埃里希氏菌主要免疫反应性糖蛋白（gp200）的B细胞表位由碳水化合物和肽决定簇定义
6. Safety specificity and immunogenicity of a PrPSc-specific prion vaccine based on the YYR disease specific epitope [O] . Ryan Taschuk, Kristen Marciniuk, Pekka Määttänen, 2014

机译：基于YYR疾病特异性表位的PrPSc特异性病毒疫苗的安全性特异性和免疫原性
7. Identification of a Glycosylated Ehrlichia canis 19-Kilodalton Major Immunoreactive Protein with a Species-Specific Serine-Rich Glycopeptide Epitope▿ [O] . McBride, Jere W., Doyle, C. Kuyler, Zhang, Xiaofeng, 2006

机译：鉴定具有特定物种丝氨酸丰富的糖肽表位的糖基化犬埃里希犬19-Kilodalton主要免疫反应蛋白▿
8. Immunoreactivity, Epitope Mapping and Protection Studies with Anti-Conotoxin GISera and Various Conotoxins [R] . Stiles, B. G., Sexton, F. W. 1992

机译：抗芋螺毒素和各种芋螺毒素的免疫反应，表位作图和保护研究

Immunoreactivity of specific epitopes of PrPSc is enhanced by pretreatment in a hydrated autoclave.

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