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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease
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Detection by Immunofluorescence Assay of Bartonella henselae in Lymph Nodes from Patients with Cat Scratch Disease

机译：猫刮擦病患者淋巴结中半夏巴尔通体的免疫荧光检测

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摘要

Laboratory diagnosis of Bartonella henselae infections can be accomplished by serology or PCR assay on biopsy samples. The purpose of our work was to assess immunofluorescence detection (IFD) in lymph node smears using a specific monoclonal antibody directed against B. henselae and a commercial serology assay (IFA) compared with PCR detection. Among 200 lymph nodes examined from immunocompetent patients, 54 were positive for B. henselae by PCR, of which 43 were also positive by IFD. Among the 146 PCR-negative lymph nodes, 11 were positive by IFD. Based on PCR results, the specificity of this new technique was 92.5%, the sensitivity was 79.6%, and the positive predictive value was 79.6%. At a cutoff titer of 64, the sensitivity of the IFA was 86.8% and the specificity was 74.1%. Diagnosis of cat scratch disease (CSD) may be improved, with a specificity of 100%, when the two tests (IFD and IFA) were negative; the sensitivity was 97.4% if one of the two tests was positive. Since PCR-based detection with biopsy samples is available only in reference laboratories, we suggest using IFD coupled with the commercial serology test for the diagnosis of CSD.
机译：可以通过对活检样本进行血清学或PCR分析来完成对汉氏巴尔通体感染的实验室诊断。我们的工作目的是评估针对淋巴结涂片的免疫荧光检测（IFD），该检测使用针对B. henselae的特异性单克隆抗体和与PCR检测相比的商业血清学检测（IFA）。在有免疫能力的患者检查的200个淋巴结中，有54例通过PCR检测出对汉森芽孢杆菌呈阳性，其中有43例也经IFD呈阳性。在146个PCR阴性淋巴结中，有11个通过IFD检测为阳性。根据PCR结果，该新技术的特异性为92.5％，灵敏度为79.6％，阳性预测值为79.6％。截止滴度为64时，IFA的灵敏度为86.8％，特异性为74.1％。当两个测试（IFD和IFA）均为阴性时，猫抓伤病（CSD）的诊断可以得到改善，特异性为100％。如果两个测试之一为阳性，则灵敏度为97.4％。由于仅在参考实验室中可以使用活检样品进行基于PCR的检测，因此我们建议将IFD与商业血清学检测结合使用以诊断CSD。

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