• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >Use of Serum or Buffer-Changed EDTA-Plasma in a Rapid Inexpensive and Easy-To-Perform Hemolytic Complement Assay for Differential Diagnosis of Systemic Lupus Erythematosus and Monitoring of Patients with the Disease
【2h】

Use of Serum or Buffer-Changed EDTA-Plasma in a Rapid Inexpensive and Easy-To-Perform Hemolytic Complement Assay for Differential Diagnosis of Systemic Lupus Erythematosus and Monitoring of Patients with the Disease

机译：在快速廉价且易于执行的溶血补体分析中使用血清或缓冲液改变的EDTA-血浆用于系统性红斑狼疮的鉴别诊断和疾病患者的监测

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We previously described a simplified quantitative hemolytic assay for classical pathway (CP) hemolytic function in serum that has been shown to correlate with the 50% hemolytic complement (CH50) assay. In the present study, we used this assay to compare CP functions; plasma levels of C3, C4, and C3dg; and ratios of C3dg to C3 in healthy individuals and patients with systemic lupus erythematosus (SLE) or rheumatoid arthritis (RA) with different degrees of complement activation. A significant depression in CP function and levels of C4 and C3 and increased C3dg levels and C3dg/C3 ratios were observed in the SLE patients. In patients with RA, CP function was normal, whereas C3, C4, and C3dg levels and the C3dg/C3 ratio were elevated. The SLE results are compatible with systemic complement consumption, whereas the RA data suggest an acute-phase reaction with a normal C3 catabolic rate. To facilitate the handling of patient samples, we also developed a method to restore the hemolytic function of EDTA-plasma by transferring it to Veronal-buffered saline containing the thrombin inhibitor lepirudin. This process inhibits coagulation and enables complement activation, allowing a longer time lag between sample harvesting and testing. These results, combined with previous correlation studies, suggest that the CP hemolytic assay can effectively replace the CH50 assay for routine SLE differential diagnosis and monitoring of disease activity.
机译：我们先前描述了一种简化的定量溶血测定法，用于血清中的经典途径（CP）溶血功能，已证明与50％溶血补体（CH50）测定法相关。在本研究中，我们使用此测定法比较CP功能。 C3，C4和C3dg的血浆水平;健康个体和系统性红斑狼疮（SLE）或类风湿性关节炎（RA）不同程度补体激活的患者中C3dg与C3的比率在SLE患者中观察到CP功能和C4和C3水平显着降低，C3dg水平和C3dg / C3比率增加。在RA患者中，CP功能正常，而C3，C4和C3dg水平以及C3dg / C3比升高。 SLE的结果与全身补体的摄入量相符，而RA数据表明急性期反应的C3分解代谢速率正常。为了方便患者样品的处理，我们还开发了一种方法，可通过将EDTA血浆的溶血功能转移到含有凝血酶抑制剂lerudrudin的Veronal缓冲盐水中来恢复其溶血功能。此过程抑制了凝结并激活了补体，从而在样品采集和测试之间存在更长的时间间隔。这些结果与先前的相关性研究相结合，表明CP溶血测定可有效替代CH50测定，用于常规SLE鉴别诊断和疾病活动监测。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号