Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection

机译：费氏衣原体CF0218是一种新型TMH家族蛋白具有潜在的诊断能力可用于诊断费氏衣原体感染

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Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.

机译：猫衣原体是猫急性和慢性结膜炎和肺炎（猫衣原体病）的病原体。另外，尽管人们对此有争议，但假单胞菌还是人类非沙眼衣原体结膜炎等疾病的人畜共患病原体。目前，尚无可方便地特异性检测猫屎肠球菌感染的血清诊断系统。当前的系统使用与所有衣原体物种交叉反应的抗原，例如脂多糖。另外，很难区分接种了针对猫屎肠球菌的商业疫苗的猫和感染了猫屎肠球菌的猫。在这里，我们描述了一种新的候选诊断抗原，用于诊断猫屎肠球菌感染，即CF0218，该抗原是通过用猫屎肠球菌免疫血清筛选猫屎肠球菌Fe / C-56的基因组表达文库而获得的。 CF0218是一种假定的跨膜头（TMH）家族蛋白，在其N端带有双叶疏水基序，在肺炎衣原体或沙眼衣原体基因组中未发现CF0218的直向同源物。重组CF0218未被抗沙眼衣原体的抗血清所识别，这表明CF0218是猫屎肠衣特异性的。通过反转录-PCR证实了猫屎肠球菌感染过程中的CF0218转录。通过间接免疫荧光分析，CF0218与被感染梭状芽孢杆菌形成的包涵体共定位。在猫屎肠球菌感染后，针对CF0218的抗体反应有所提高，但在实验接种和感染的猫中却没有接种疫苗。这些结果表明，CF0218是 C的新型TMH家族蛋白。 felis ，具有作为 C的潜力。 felis 感染特异性诊断抗原。 展开▼

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期刊名称 Clinical and Diagnostic Laboratory Immunology

作者
Kenji Ohya; Yu Takahara; Etsuko Kuroda; Saori Koyasu; Shigeyuki Hagiwara; Maki Sakamoto; Mitsuaki Hisaka; Kazuko Morizane; Shinryou Ishiguro; Tsuyoshi Yamaguchi; Hideto Fukushi;
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作者单位

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年(卷),期 2008(15),10

年度 2008

页码 1606–1615

总页数 10

原文格式 PDF

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中图分类免疫学;

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专利

1. Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection [J] . Kenji Ohya, Yu Takahara, Etsuko Kuroda, Clinical and vaccine immunology: CVI . 2008,第10期

机译：费氏衣原体CF0218是一种新型TMH家族蛋白，具有潜在的诊断抗原，可用于诊断费氏衣原体感染

2. Treatment and management of Chlamydophila felis infections in cats.Original Title Chlamydophila-felis-Infektion bei Katzen. [J] . Hartmann A., Hartmann K. Tierarztliche Praxis, Ausgabe K. Kleintiere . 2010,第4期

机译：猫的猫嗜衣藻的感染的治疗和管理。原标题为卡岑的猫嗜衣藻的感染。

3. Treatment and management of Chlamydophila felis infections in cats.Original Title Chlamydophila-felis-Infektion bei Katzen. [J] . Hartmann A., Hartmann K. Tierarztliche Praxis, Ausgabe K. Kleintiere . 2010,第4期

机译：猫衣衣的治疗和治疗和治疗猫的感染。患者感染。原冠状题衣原体Chlamydophila-Felis -infektion Bei Katzen。

4. ENVIRONMENTAL ASSAY TO MEASURE CAT FLEA (CTENOPHALIDES FELIS) ANTIGENS IN DUST SAMPLES USING POLYCLONAL ANITBODIES [C] . R Codina, R Diwadkar, R Lockey International conference on indoor air quality and climate . 2002

机译：使用多克隆抗体测量粉尘样品中的猫跳蚤（Ctenocephalides Felis）抗原的环境测定

5. Haemobartonella felis (Mycoplasma haemofelis) molecular diagnostics and genomic studies. [D] . Berent, Linda Marie. 2002

机译：血型杆状杆菌（Mycoplasma haemofelis）分子诊断和基因组研究。

6. Comparison of polymerase chain reaction tests for diagnosis of feline herpesvirus Chlamydophila felis and Mycoplasma spp. infection in cats with ocular disease in Canada [O] . Lynne S. Sandmeyer, Cheryl L. Waldner, Bianca S. Bauer, 2010

机译：用于诊断猫疱疹病毒猫衣原体和支原体的聚合酶链反应测试的比较。在加拿大患有眼病的猫感染

7. Chlamydophila felis CF0218 is a novel TMH family protein with potential as a diagnostic antigen for diagnosis of C. felis infection [O] . OHYA, Kenji, TAKAHARA, Yu, KURODA, Etsuko, 2008

机译：费氏衣原体CF0218是一种新型TMH家族蛋白，有可能作为诊断抗原来诊断费氏衣原体感染

8. Human Infection with Rickettsia felis, Kenya [R] . Richards, A. L., Jiang, J., Omulo, S., 2010

机译：人类感染与立克次体猫科动物，肯尼亚

1. 抗肺炎衣原体蛋白酶样活性因子单克隆抗体在冠状动脉硬化患者肺炎衣原体感染诊断中的初步应用 [J] . 唐熟能 ,郑江花 ,莫小辉 . 中国动脉硬化杂志 . 2013,第4期

2. 赵孟頫与浙西航海家族的交游——以昆山顾氏、朱氏及长兴费氏为例 [J] . 陈波 . 元史及民族与边疆研究集刊 . 2018,第002期

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4. 橘小实蝇一种重要的寄生蜂-凡氏费氏茧蜂 [J] . 章玉苹 ,赵远超 ,张宝鑫 . 环境昆虫学报 . 2008,第001期

5. 獭兔疑似多杀性费朗西斯氏菌病的诊断与治疗 [J] . 周振银 . 中国养兔杂志 . 1996,第005期

6. 在费氏旋转台上测定石英光轴空间方位的一种新方法 [C] . 金玉书 . 全国显微构造与组构学术讨论会 . -1

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1. 一种具有沙眼衣原体感染诊断和/或预后评估的试剂盒 [P] . 中国专利： CN112899377A . 2021-06-04

2. 用于治疗和诊断衣原体感染的化合物和方法 [P] . 中国专利： CN100467600C . 2009.03.11

3. NOVEL FELIS IMMUNODEFICIENCY VIRUS STRAIN AND DNA CORRESPONDING TO THE RNA OF ITS ENVELOP PROTEIN GENE AND FELIS IMMUNODEFICIENCY VIRUS VACCINE [P] . 外国专利： JPH07236476A . 1995-09-12

机译：新型猫免疫缺陷病毒株及其与其包膜蛋白基因和肠球菌免疫缺陷病毒RNA对应的DNA。

4. antigenic polypeptide composition, family of purified genes, family of polynucleotides, plasmodium falciparum gene isolated from a family, polynucleotide sequence, protein, antigenic polypeptide, immunogenic compositions, vaccines, use of a recombinant protein, purified recombinant or synthetic fragment antibody antibody, antibody set or antibody fragment, use of a composition, medicament, diagnostic methods, diagnostic kits, recombinant nucleotide sequence, recombinant expression and / or cloning vector, use of a recombinant expression cell and expression vector [P] . 外国专利： BRPI0415784A . 2006-12-26

机译：抗原多肽组成，纯化基因家族，多核苷酸家族，从家族分离的恶性疟原虫基因，多核苷酸序列，蛋白质，抗原多肽，免疫原性组合物，疫苗，重组蛋白的用途，纯化的重组或合成片段抗体抗体，抗体组或抗体片段，组合物的用途，药物，诊断方法，诊断试剂盒，重组核苷酸序列，重组表达和/或克隆载体，重组表达细胞和表达载体的用途

5. Isolated negative-sense single stranded rna virus, isolated or recombinant nucleic acid or its specific functional mpv fragment, vector, host cell, isolated or recombinant proteinaceous molecule or its specific functional mpv fragment, antigen, antibody, method for identifying a viral isolate as a mpv, viral isolate, methods for virologically and serologically diagnosing a mammalian mpv infection, diagnostic kit for diagnosing a mpv infection, uses of a virus, a nucleic acid, a vector, a cell host, a proteinaceous molecule or fragment thereof, an antigen, or an antibody, pharmaceutical composition, methods for treating or preventing an mpv infection and respiratory disease, and for obtaining an antiviral agent useful in the treatment of disease. of the respiratory tract, antiviral agent, use of a viral agent, methods for virologically diagnosing and serum logically a bird apv infection, use of a diagnostic test, and method for detecting an antibody directed against mpv in a sample [P] . 外国专利： BR0206591A . 2005-02-01

机译：分离的负义单链RNA病毒，分离的或重组的核酸或其特定功能的mpv片段，载体，宿主细胞，分离的或重组的蛋白质分子或其特定的功能mpv片段，抗原，抗体，将病毒分离物鉴定为mpv，病毒分离物，用于病毒学和血清学诊断哺乳动物mpv感染的方法，用于诊断mpv感染的诊断试剂盒，病毒，核酸，载体，细胞宿主，蛋白质分子或其片段，抗原的用途，或抗体，药物组合物，用于治疗或预防mpv感染和呼吸道疾病以及获得可用于治疗疾病的抗病毒剂的方法。呼吸道，抗病毒药，病毒药的使用，禽apv感染的病毒学诊断和逻辑诊断方法，诊断测试的使用以及检测样品中针对mpv的抗体的方法

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Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection

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