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首页> 美国卫生研究院文献>Clinical and Diagnostic Laboratory Immunology >IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer)
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IP-10 Is a Sensitive Biomarker of Antigen Recognition in Whole-Blood Stimulation Assays Used for the Diagnosis of Mycobacterium bovis Infection in African Buffaloes (Syncerus caffer)

机译：IP-10是用于非洲水牛（Syncerus caffer）牛分枝杆菌感染诊断的全血刺激测定法中抗原识别的敏感生物标记。

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African buffaloes (Syncerus caffer) are maintenance hosts of Mycobacterium bovis, the causative agent of bovine tuberculosis. They act as reservoirs of this infection for a wide range of wildlife and domestic species, and the detection of infected animals is important to control the geographic spread and transmission of the disease. Interferon gamma (IFN-γ) release assays (IGRAs) utilizing pathogen-derived peptide antigens are highly specific tests of M. bovis infection; however, the diagnostic sensitivities of these assays are suboptimal. We evaluated the diagnostic utility of measuring antigen-dependent interferon gamma-induced protein 10 (IP-10) release as an alternative to measuring IFN-γ levels. M. bovis-exposed buffaloes were tested using the Bovigam PC-EC and Bovigam PC-HP assays and a modified QuantiFERON TB-Gold (mQFT) assay. IP-10 was measured in the harvested plasma and was produced in significantly greater abundance in response to M. bovis antigens in Bovigam-positive than in Bovigam-negative animals. For each assay, using the Bovigam results as a reference, receiver operating characteristic curve analysis was done to determine diagnostically relevant cutoff values for IP-10. Thereafter, mQFT test results derived from measurement of IP-10 and IFN-γ were compared and a larger number of Bovigam-positive animals were detected using IP-10 as a diagnostic marker. Moreover, using IP-10, agreement between the mQFT assay and the Bovigam assays was increased, while the excellent agreement between the Bovigam assays was retained. We conclude that IP-10 is a sensitive marker of antigen recognition and that measurement of this cytokine in antigen-stimulated whole blood might increase the sensitivity of conventional IGRAs in African buffaloes.
机译：非洲水牛（Syncerus caffer）是牛结核分枝杆菌的维持宿主，牛结核分枝杆菌是牛结核的病原体。它们充当了广泛的野生动植物和家养物种感染的储存库，对感染动物的检测对于控制疾病的地理传播和传播非常重要。利用病原体衍生的肽抗原的干扰素γ（IFN-γ）释放测定法（IGRA）是牛分枝杆菌感染的高度特异性检测。然而，这些测定法的诊断敏感性不是最佳的。我们评估了测量抗原依赖性干扰素γ诱导的蛋白10（IP-10）释放作为测量IFN-γ水平的替代方法的诊断实用性。使用Bovigam PC-EC和Bovigam PC-HP测定法和改进的QuantiFERON TB-Gold（mQFT）测定法测试了牛分枝杆菌暴露的水牛。在收获的血浆中测量IP-10，与Bovigam阴性动物相比，在Bovigam阳性中对牛分枝杆菌抗原产生的响应丰度明显更高。对于每个测定，均以Bovigam结果作为参考，进行接收器工作特性曲线分析，以确定IP-10的诊断相关临界值。此后，比较了从IP-10和IFN-γ的测量得出的mQFT测试结果，并使用IP-10作为诊断标记物检测了大量Bovigam阳性动物。此外，使用IP-10，可以提高mQFT分析与Bovigam分析之间的一致性，同时保留Bovigam分析之间的出色一致性。我们得出的结论是IP-10是抗原识别的敏感标记，并且在抗原刺激的全血中测量该细胞因子可能会增加非洲水牛中常规IGRA的敏感性。

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