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首页> 美国卫生研究院文献>Clinical Molecular Pathology >Investigation of tissue organelles by a combination of analytical subcellular fractionation and enzymic microanalysis: a new approach to pathology.
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Investigation of tissue organelles by a combination of analytical subcellular fractionation and enzymic microanalysis: a new approach to pathology.

机译：通过分析亚细胞分级分离和酶微分析相结合的方法研究组织细胞器：一种新的病理学方法。

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Classical approaches to the study of pathological tissue have relied mainly on morphological techniques. In an attempt to quantitate the abnormalities and to investigate the pathogenesis of tissue disorders at a subcellular level we have combined analytical subcellular fractionation by sucrose density gradient centrifugation with microanalysis of tissue enzymic activities. The methodological problems of performing these studies on milligram quantities of tissue are discussed. Details of the appropriate equipment are provided, and its application to the study of human liver specimens is described. As an example of this approach, biochemical and subcellular fractionation experiments on tissue from patients with both primary and secondary hepatic lysosomal storage diseases are discussed. Examination of the lysosomal changes reveals that increased enzyme activity is a common finding in these disorders but tissue damage occurs only when there is evidence of enhances lysosomal fragility with intracellular release of degradative enzymes. Other tissues which have proved amenable to study in this manner and in which profitable results in the investigation of their disorders have been obtained are listed.
机译：研究病理组织的经典方法主要依靠形态学技术。为了量化异常并研究亚细胞水平的组织疾病的发病机理，我们将通过蔗糖密度梯度离心法进行的亚细胞分馏分析与组织酶活性的微分析相结合。讨论了对毫克量的组织进行这些研究的方法学问题。提供了适当设备的详细信息，并介绍了其在人类肝脏标本研究中的应用。作为这种方法的一个例子，讨论了患有原发性和继发性肝溶酶体贮积病患者的组织的生化和亚细胞分级实验。溶酶体变化的检查表明，增加的酶活性是这些疾病的常见发现，但是只有在有证据表明溶酶体的脆性随细胞内释放降解酶而增强时，组织损伤才会发生。列出了其他证明可以进行这种研究的组织，并已获得了对其疾病进行研究的有益结果。

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