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The Impact of Human Activities on Microbial Quality of Rivers in the Vhembe District South Africa

机译:人类活动对南非Vhembe区河流微生物质量的影响

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摘要

>Background: Water quality testing is dictated by microbial agents found at the time of sampling in reference to their acceptable risk levels. Human activities might contaminate valuable water resources and add to the microbial load present in water bodies. Therefore, the effects of human activities on the microbial quality of rivers collected from twelve catchments in the Vhembe District in South Africa were investigated, with samples analyzed for total coliform (TC) and Eschericha coli (E. coli) contents. >Methods: Physical parameters and various human activities were recorded for each sampling site. The Quanti-Tray® method was adopted for the assessment of TC and E. coli contents in the rivers over a two-year period. A multiplex polymerase chain (PCR) method was used to characterize the strains of E. coli found. >Results: The microbial quality of the rivers was poor with both TC and E. coli contents found to be over acceptable limits set by the South African Department of Water and Sanitation (DWS). No significant difference (p > 0.05) was detected between TC and E. coli risks in dry and wet seasons. All six pathogenic E. coli strains were identified and Enteroaggregative E. coli (EAEC), atypical Enteropathogenic E. coli (a-EPEC) and Enterotoxigenic E. coli (ETEC) were the most prevalent E. coli strains detected (respectively, 87%, 86% and 83%). >Conclusions: The study indicated that contamination in the majority of sampling sites, due to human activities such as car wash, animal grazing and farming, poses health risks to communities using the rivers for various domestic chores. It is therefore recommended that more education by the respective departments is done to avert pollution of rivers and prevent health risks to the communities in the Vhembe District.
机译:>背景:水质测试是由采样时发现的微生物剂参考可接受的风险水平决定的。人类活动可能会污染宝贵的水资源,并增加水体中存在的微生物负荷。因此,调查了人类活动对南非Vhembe区12个流域收集的河流微生物质量的影响,并分析了样品中的总大肠菌群(TC)和大肠杆菌(E. coli)含量。 >方法:记录每个采样点的身体参数和各种人类活动。采用Quanti-Tray ®方法评估两年中河流中TC和大肠杆菌的含量。多重聚合酶链(PCR)方法用于表征发现的大肠杆菌菌株。 >结果:由于TC和E.coli含量均超出南非水与卫生部(DWS)设定的可接受范围,河流的微生物质量较差。在干燥和潮湿季节,TC和大肠杆菌风险之间未发现显着差异(p> 0.05)。鉴定出所有六种致病性大肠杆菌菌株,其中检出的肠聚集性大肠杆菌(EAEC),非典型肠致病性大肠杆菌(a-EPEC)和肠毒素性大肠杆菌(ETEC)是检出率最高的大肠杆菌菌株(分别为87% ,86%和83%)。 >结论:研究表明,由于人类活动(例如洗车,放牧和耕种),大多数采样点的污染对使用河流进行各种家务劳动的社区构成了健康风险。因此,建议各部门进行更多的教育,以防止河流污染和防止对Vhembe区社区的健康危害。

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