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首页> 美国卫生研究院文献>International Journal of Environmental Research and Public Health >Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods
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Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods

机译：使用文化和生物分子方法监测不同生物能源设施中的空气微生物污染

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摘要

Bioaerosol exposure linked to the bioenergy production from waste and its effects on human health in occupational and residential environments has rising interest nowadays. The health risk associated with the exposure includes mainly infective diseases, allergies, chronic bronchitis, and obstructive pulmonary disease. A risk assessment’s critical point is the bioaerosol quality and quantity characterization. The aim of this study is to evaluate the application of different methods for the analysis of bioaerosol sampled in bioenergy plants. This study involved six Italian plants for the treatment of biomasses and energy production. Bioaerosol cultural evaluation was performed, by Surface Air System (SAS) sampler, and DNA was extracted from PM0.49 samples and Low Melting Agar plates. qRT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and band sequencings were performed. The cultural method is able to detect less than 15% of what is evaluable with bio-molecular methods. A low sample alfa-diversity and a high beta-biodiversity in relation to feedstock and process were observed. Sequencing showed microorganisms with a hygienic-sanitary relevance such as Arcobacter, Pseudomonas, Enterobacter, Klebsiella, Enterococcus and Bacillus. Integrated cultural and biomolecular methods can be more exhaustive to evaluate bioaerosol’s exposure in the occupational environment.
机译：如今，与悬浮物产生的生物能有关的生物气溶胶暴露及其在职业和居住环境中对人类健康的影响日益引起人们的关注。与接触有关的健康风险主要包括传染病，过敏，慢性支气管炎和阻塞性肺部疾病。风险评估的关键点是生物气溶胶的质量和数量表征。这项研究的目的是评估不同方法在生物能源工厂采样的生物气溶胶分析中的应用。这项研究涉及六家意大利工厂，用于处理生物质和能源生产。通过地面空气系统（SAS）采样器进行生物气溶胶培养评估，并从PM0.49样品和低熔点琼脂平板中提取DNA。进行qRT-PCR，然后进行变性梯度凝胶电泳（DGGE）和条带测序。文化方法只能检测不到生物分子方法可评估值的15％。观察到相对于原料和工艺而言，样品的α-生物多样性较低，而β-生物多样性较高。测序显示了具有卫生卫生意义的微生物，如弓形杆菌，假单胞菌，肠杆菌，克雷伯菌，肠球菌和芽孢杆菌。综合的文化和生物分子方法可以更详尽地评估生物气溶胶在职业环境中的暴露情况。

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