Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu

机译：海洋芽孢杆菌的系统发育框架和生物表面活性剂基因表达分析。泰米尔纳德邦东部沿海平原

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The present study emphasizes the diversity assessment of marine Bacillus species with special reference to biosurfactant production, respective gene expression, and discrimination among Bacillus licheniformis and Bacillus subtilis. Among the 200 individual species of eastern coastal plain of Tamil Nadu screened, five biosurfactant producing potential bacterial species with entirely different morphology were selected. Biochemical and 16S rRNA gene sequence analysis suggested that all the said five species belong to Bacillus genera but differ in species levels. Biosurfactant of all the five species fluctuates in greater levels with respect to activity as well as to constituents but showed partial similarity to the commercially available surfactin. The expression of srf gene was realized in all of the five species. However, the sfp gene expression was observed only in three species. In conclusion, both B. licheniformis and B. subtilis demonstrate srf gene; nevertheless, sfp gene was expressed only by Bacillus subtilis.

机译：本研究强调海洋芽孢杆菌物种的多样性评估，特别要注意生物表面活性剂的产生，各自的基因表达以及地衣芽孢杆菌和枯草芽孢杆菌之间的区别。在筛选的泰米尔纳德邦东部沿海平原的200个个体物种中，选择了5种产生表面活性剂的潜在生物菌种，它们的形态完全不同。生化和16S rRNA基因序列分析表明，上述五个物种全部属于芽孢杆菌属，但物种水平不同。所有这五个物种的生物表面活性剂在活性和成分方面都有较大的波动，但与市售表面活性剂存在部分相似性。 srf基因的表达在这五个物种中都实现了。但是，仅在三个物种中观察到了sfp基因表达。总之，地衣芽孢杆菌和枯草芽孢杆菌均显示srf基因。但是，sfp基因仅由枯草芽孢杆菌表达。

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期刊名称 International Journal of Bacteriology
作者
Sreethar Swaathy; Varadharajan Kavitha; Arockiasamy Sahaya Pravin; Ganesan Sekaran; Asit Baran Mandal; Arumugam Gnanamani;
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年(卷),期 2014(2014),-1
年度 2014
页码 860491
总页数 10
原文格式 PDF
正文语种
中图分类微生物学;
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机译：海洋芽孢杆菌的系统发育框架和生物表面活性剂基因表达分析。泰米尔纳德邦东部沿海平原
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Phylogenetic Framework and Biosurfactant Gene Expression Analysis of Marine Bacillus spp. of Eastern Coastal Plain of Tamil Nadu

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