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首页> 美国卫生研究院文献>Microbial Cell Factories >A comparative study: the impact of different lipid extraction methods on current microalgal lipid research
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A comparative study: the impact of different lipid extraction methods on current microalgal lipid research

机译：比较研究：不同脂质提取方法对当前微藻脂质研究的影响

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Microalgae cells have the potential to rapidly accumulate lipids, such as triacylglycerides that contain fatty acids important for high value fatty acids (e.g., EPA and DHA) and/or biodiesel production. However, lipid extraction methods for microalgae cells are not well established, and there is currently no standard extraction method for the determination of the fatty acid content of microalgae. This has caused a few problems in microlagal biofuel research due to the bias derived from different extraction methods. Therefore, this study used several extraction methods for fatty acid analysis on marine microalga Tetraselmis sp. M8, aiming to assess the potential impact of different extractions on current microalgal lipid research. These methods included classical Bligh & Dyer lipid extraction, two other chemical extractions using different solvents and sonication, direct saponification and supercritical CO2 extraction. Soxhlet-based extraction was used to weigh out the importance of solvent polarity in the algal oil extraction. Coupled with GC/MS, a Thermogravimetric Analyser was used to improve the quantification of microalgal lipid extractions. Among these extractions, significant differences were observed in both, extract yield and fatty acid composition. The supercritical extraction technique stood out most for effective extraction of microalgal lipids, especially for long chain unsaturated fatty acids. The results highlight the necessity for comparative analyses of microalgae fatty acids and careful choice and validation of analytical methodology in microalgal lipid research.
机译：微藻细胞具有迅速积聚脂质的潜力，例如含有对高价值脂肪酸（例如，EPA和DHA）和/或生物柴油生产重要的脂肪酸的三酰基甘油酯。然而，用于微藻细胞的脂质提取方法尚未很好地建立，并且目前没有用于确定微藻的脂肪酸含量的标准提取方法。由于来自不同提取方法的偏差，这在微滨海生物燃料研究中引起了一些问题。因此，本研究使用了几种提取方法对海洋微藻Tetraselmis sp。进行脂肪酸分析。 M8，旨在评估不同提取物对当前微藻脂质研究的潜在影响。这些方法包括经典的Bligh＆Dyer脂质提取，使用不同溶剂和超声处理的其他两种化学提取，直接皂化和超临界CO2提取。基于索氏提取的方法用来衡量藻油提取中溶剂极性的重要性。结合GC / MS，使用热重分析仪改善微藻脂质提取物的定量。在这些提取物中，提取率和脂肪酸组成均观察到显着差异。超临界萃取技术最有效地萃取微藻类脂质，特别是长链不饱和脂肪酸。结果强调了微藻脂肪酸比较分析的必要性，以及在微藻脂质研究中仔细选择和验证分析方法的必要性。

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