• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Insects >Efficacy of Topical Application Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations
【2h】

Efficacy of Topical Application Leaf Residue or Soil Drench of Blastospores of Isaria fumosorosea for Citrus Root Weevil Management: Laboratory and Greenhouse Investigations

机译：伊斯利亚扁桃体芽孢的局部施用叶片残留或土壤淋湿对柑橘根象鼻虫管理的功效：实验室和温室调查

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The efficacy of topical, leaf residue, and soil drench applications with Isaria fumosorosea blastospores (Ifr strain 3581) was assessed for the management of the citrus root weevil, Diaprepes abbreviatus (L.). Blastospores of Ifr were applied topically at a rate of 107 blastospores mL−1 on both the larvae and adults, and each insect stage was incubated in rearing cups with artificial diet at 25 °C, either in the dark or in a growth chamber under a 16 h photophase for 2 weeks, respectively. Percent larval and adult mortality due to the infection of Ifr was assessed after 14 days as compared to untreated controls. Leaf residue assays were assessed by feeding the adults detached citrus leaves previously sprayed with Ifr (107 blastospores mL−1) in Petri dish chambers and then incubating them at 25 °C for 2–3 weeks. Efficacy of the soil drench applications was assessed on five larvae feeding on the roots of a Carrizo hybrid citrus seedling ~8.5–10.5 cm below the sterile sand surface in a single 16 cm × 15.5 cm pot inside a second pot lined with plastic mesh to prevent escapees. Drench treatments per pot consisted of 100 mL of Ifr suspension (107 blastospores mL−1), flushed with 400, 900, or 1400 mL of water compared to 500, 1000, and 1500 mL of water only for controls. The mean concentration of Ifr propagules as colony forming units per gram (CFUs g−1) that leached to different depths in the sand profile per treatment drench rate was also determined. Two weeks post-drenching of Ifr treatments, larvae were assessed for percent mortality, size differences, and effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. Topical spray applications caused 13 and 19% mortality in larvae and adults after 7 days compared to none in the control after 14 days, respectively. Adults feeding on a single Ifr treated leaf for 24 h consumed less than the control, and resulted in 100% mortality 35 days post-treatment compared to 33% in the untreated control. Although offered fresh, untreated leaves after 24 h, only adults in the control group consumed them. Ifr CFUs g−1 were isolated 8.5–10.5 cm below the sand surface for the 1000 and 1500 mL drench rates only, resulting in 2%–4% larval mortality. For all the Ifr drench treatments, no differences were observed in percent larval mortality and size or the effect of treatments in reducing feeding damage to the plant root biomass compared to the controls. These results suggest that the foliar application of Ifr may be an efficient biocontrol strategy for managing adult populations of D. abbreviatus; potential alternative larval management strategies are discussed.
机译：评估了Isaria fumosorosea blastospores（Ifr株3581）局部，叶面残留和土壤淋湿对柑橘根象鼻虫Diaprepes abbreviatus（L.）的管理效果。在幼虫和成虫上均以10 7 芽孢菌mL −1 的比例局部施用Ifr的芽孢菌，将每个昆虫阶段在人工喂养的饲养杯中于90℃孵育。 25°C，在黑暗中或在16 h光相下的生长室中分别放置2周。与未治疗的对照组相比，评估了14天后因Ifr感染引起的幼虫百分率和成年死亡率。通过在培养皿室中饲喂预先喷洒了Ifr（10 7 芽孢杆菌mL -1 ）的成年离体柑橘叶片，然后在25°C下孵育，来评估叶片残留量2-3周。在五个无菌幼虫的根上，在不育的沙子表面下方约8.5-10.5 cm处的一个Carrizo杂种柑橘幼苗的根部上，取食了五个幼虫，在一个单独的16 cm×15.5 cm的盆中，第二个盆内衬塑料网以防止逃脱者。每盆淋水处理由100 mL的Ifr悬浮液（10 7 芽孢子mL −1 ）组成，用400、900或1400 mL的水冲洗，而500、1000的水冲洗，以及仅用于对照的1500 mL水。还确定了每处理湿润率浸出到砂剖面不同深度的每克菌落形成单位每克Ifr繁殖体的平均浓度（CFUs g -1 ）。与对照相比，在Ifr处理的浸泡后两周，评估了幼虫的死亡率百分比，大小差异以及处理在减少对根系生物量的进食损害方面的效果。 7天后局部喷洒可导致幼虫和成虫的死亡率分别为13％和19％，而14天后对照组则没有。以单只Ifr处理的叶子饲养24小时的成年动物消耗的脂肪比对照组少，导致处理后35天的死亡率为100％，而未处理的对照组为33％。尽管24小时后提供了未经处理的新鲜叶子，但对照组中只有成年人食用了它们。仅对于1000和1500 mL的淋湿率，在砂面以下8.5–10.5 cm处分离出Ifr CFU g -1 ，导致幼虫死亡率为2％–4％。与对照相比，对于所有的Ifr淋水处理，未观察到幼虫死亡率百分比和大小或处理在减少对植物根生物量的进食损害方面的效果方面的差异。这些结果表明，叶面施用Ifr可能是一种有效的生物防治策略，用于管理成年的D. abbreviatus种群。讨论了潜在的替代幼虫管理策略。

著录项

相似文献

  • 外文文献
  • 中文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号