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L-lactic acid production by Aspergillus brasiliensis overexpressing the heterologous ldha gene from Rhizopus oryzae

机译:巴西曲霉过表达米根霉异源ldha基因的L-乳酸生产

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摘要

BackgroundLactic acid is the building block of poly-lactic acid (PLA), a biopolymer that could be set to replace petroleum-based plastics. To make lactic acid production cost-effective, the production process should be carried out at low pH, in low-nutrient media, and with a low-cost carbon source. Yeasts have been engineered to produce high levels of lactic acid at low pH from glucose but not from carbohydrate polymers (e.g. cellulose, hemicellulose, starch). Aspergilli are versatile microbial cell factories able to naturally produce large amounts of organic acids at low pH and to metabolize cheap abundant carbon sources such as plant biomass. However, they have never been used for lactic acid production.
机译:背景技术乳酸是聚乳酸(PLA)的基础,聚乳酸是一种生物聚合物,可以替代石油基塑料。为了使乳酸生产具有成本效益,生产过程应在低pH值,低营养介质和低成本碳源下进行。酵母经过改造后可以在低pH下从葡萄糖中产生高水平的乳酸,而不是从碳水化合物聚合物(例如纤维素,半纤维素,淀粉)中产生高水平的乳酸。曲霉菌是多功能的微生物细胞工厂,能够在低pH值下自然产生大量有机酸,并代谢廉价的丰富碳源,例如植物生物质。但是,它们从未用于乳酸生产。

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