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首页> 美国卫生研究院文献>Infection and Immunity >Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice.
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Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice.

机译：白细胞介素1在牙周疾病中的功能作用：在C3H / HeN和C3H / HeJ小鼠腹膜巨噬细胞中牙龈杆菌细菌脂多糖诱导白细胞介素1的产生。

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Hot phenol-water-extracted lipopolysaccharide (LPS) from Bacteroides gingivalis 381 was purified by Sephadex G-100 chromatography with Tris buffer supplemented with sodium deoxycholate and EDTA (B-LPS). In the present study, B-LPS was examined for its ability to induce interleukin 1 (IL-1) production, a mitogenic response, and macrophage activation in LPS high-responder C3H/HeN and low-responder C3H/HeJ mice. A significant increase in IL-1 production was observed in C3H/HeN and C3H/HeJ peritoneal macrophages treated with various doses (1.0 to 50 micrograms/ml) of B-LPS. IL-1 production by C3H/HeN macrophages treated with B-LPS (10 micrograms/ml) was about seven times greater than that by C3H/HeJ macrophages. However, the IL-1 production induced by B-LPS (10 micrograms/ml) in C3H/HeN macrophages was four times lower compared with that induced by Escherichia coli O111 B4 LPS. Also, a significant increase in IL-1 production was found in human monocytes stimulated with B-LPS. That B-LPS-induced IL-1 exhibits some molecular weight heterogeneity was indicated from Sephadex G-75 gel filtration profiles. A significant, high mitogenic response by whole spleen cells with 1 X 10(5) to 5 X 10(4) cells of either mouse strain per well treated with B-LPS (10 to 50 micrograms/ml) was observed. However, the response of C3H/HeJ mice was less than that of the C3H/HeN strain. Also, glucose consumption assays indicated that enhanced macrophage activation occurred in C3H/HeN but not in C3H/HeJ mice treated with B-LPS. In light of recent studies showing that IL-1 stimulates bone resorption in a mouse calvaria system and collagenase production in fibroblasts, we suggest that B-LPS-induced IL-1 may play a significant role in the pathogenesis of adult periodontal disease.
机译：通过Sephadex G-100色谱法，用Tris缓冲液补充脱氧胆酸钠和EDTA（B-LPS），纯化牙龈细菌（Bacteroides gingivalis）381中的热酚水提取脂多糖（LPS）。在本研究中，检查了B-LPS在LPS高应答C3H / HeN和低应答C3H / HeJ小鼠中诱导白介素1（IL-1）产生，促有丝分裂应答和巨噬细胞活化的能力。在用各种剂量（1.0至50微克/毫升）的B-LPS处理的C3H / HeN和C3H / HeJ腹膜巨噬细胞中，IL-1的产生量显着增加。用B-LPS（10微克/毫升）处理的C3H / HeN巨噬细胞产生的IL-1大约是C3H / HeJ巨噬细胞产生的IL-1的七倍。然而，与大肠杆菌O111 B4 LPS相比，B-LPS（10微克/毫升）在C3H / HeN巨噬细胞中诱导的IL-1产量低四倍。同样，在用B-LPS刺激的人单核细胞中发现IL-1的产生显着增加。从Sephadex G-75凝胶过滤图中可以看出B-LPS诱导的IL-1表现出一定的分子量异质性。在B-LPS（10至50微克/毫升）处理的每孔中，每只小鼠品系的1 X 10（5）至5 X 10（4）细胞的整个脾脏细胞均具有明显的高促有丝分裂反应。但是，C3H / HeJ小鼠的反应小于C3H / HeN株。而且，葡萄糖消耗测定法表明增强的巨噬细胞活化发生在用B-LPS处理的C3H / HeN小鼠中，而不发生在C3H / HeJ小鼠中。根据最近的研究表明IL-1刺激小鼠颅骨系统中的骨吸收和成纤维细胞中胶原酶的产生，我们建议B-LPS诱导的IL-1可能在成人牙周病的发病机理中起重要作用。

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