首页> 美国卫生研究院文献>Infection and Immunity >The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning sequence determination and genomic organization of the gene coding for antigen 85-C of M. tuberculosis.

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The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning sequence determination and genomic organization of the gene coding for antigen 85-C of M. tuberculosis.

机译：编码结核分枝杆菌和牛分枝杆菌BCG抗原85复合物的基因是一个基因家族的成员：编码结核分枝杆菌抗原85-C的基因的克隆序列测定和基因组组织。

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摘要

A gene encoding the 33-kDa secreted protein of Mycobacterium tuberculosis (antigen 85-C) was isolated and sequenced. The corresponding DNA sequence contains a 1,020-bp coding region. The deduced amino acid sequence corresponds to a 340-residue protein consisting of a 46-amino-acid signal peptide and a 294-amino-acid mature protein. Comparison with previously described genes for the 30-kDa antigen (the alpha antigen of M. bovis BCG, also called antigen 85-B) and the 32-kDa antigens from M. bovis BCG and M. tuberculosis (antigens 85-A) indicates that the three genes share considerable sequence homology (70.8 to 77.5%) but may also code for distinctive epitopes. Strong differences among the three sequences are clearly visible upstream and downstream from the region coding for the mature proteins. The three genes have been detected in the genome of M. bovis BCG by Southern blot hybridization with three type-specific probes. Furthermore, hybridization of large DNA fragments (100 to 1,000 kbp) from M. tuberculosis separated by pulsed-field gel electrophoresis showed that the three genes coding for the antigen 85 complex are not clustered within the bacterial genome.

机译：分离并测序编码结核分枝杆菌的33kDa分泌蛋白的基因（抗原85-C）。相应的DNA序列包含一个1,020-bp的编码区。推导的氨基酸序列对应于340个残基的蛋白质，其由46个氨基酸的信号肽和294个氨基酸的成熟蛋白质组成。与先前描述的30 kDa抗原（牛分枝杆菌BCG的α抗原，也称为抗原85-B）和来自牛分枝杆菌BCG和结核分枝杆菌的32 kDa抗原（抗原85-A）的基因进行比较表明这三个基因具有相当大的序列同源性（70.8至77.5％），但也可能编码独特的表位。在编码成熟蛋白的区域的上游和下游，可以清楚地看到三个序列之间的强烈差异。通过与三种类型特异性探针的Southern印迹杂交，在牛分枝杆菌BCG的基因组中检测到了三个基因。此外，通过脉冲场凝胶电泳分离的结核分枝杆菌的大DNA片段（100至1,000 kbp）的杂交显示，编码抗原85复合物的三个基因未在细菌基因组内聚集。

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期刊名称 Infection and Immunity
作者
J Content; A de la Cuvellerie; L De Wit; V Vincent-Levy-Frébault; J Ooms; J De Bruyn;
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作者单位

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年(卷),期 1991(59),9
年度 1991
页码 3205–3212
总页数 8
原文格式 PDF
正文语种
中图分类免疫学;病毒传染病;
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专利

1. Cloning and characterization of the genes coding for antigen 85A, 85B and 85C of Mycobacterium avium subsp paratuberculosis [J] . Dheenadhayalan V., Shin KS., Chang CF., DNA Sequence: The Journal of DNA Sequencing and Mapping . 2002,第5期

机译：鸟分枝杆菌副结核菌副结核85A，85B和85C抗原编码基因的克隆和鉴定
2. Characterization of human cellular immune responses to Mycobacterium tuberculosis proteins encoded by genes predicted in RD15 genomic region that is absent in Mycobacterium bovis BCG. [J] . Al Attiyah R, Mustafa AS FEMS immunology and medical microbiology . 2010,第2期

机译：牛结核分枝杆菌BCG中不存在的RD15基因组区域中预测的基因编码的人类对结核分枝杆菌蛋白的细胞免疫应答的表征。
3. Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG [J] . Nucleic acids research . 1990,第13期

机译：牛分枝杆菌BCG的32 kDa蛋白质基因（抗原85 A）的核苷酸序列
4. EPITOPE AND AFFINITY DETERMINATION OF RECOMBINANT MYCOBACTERIUM TUBERCULOSIS AG85B ANTIGEN TOWARDS ANTI-AG85 ANTIBODIES USING PROTEOLYTIC-AFFINITY MASS SPECTROMETRY AND BIOSENSOR ANALYSIS [C] . Francesca Rinaldi, Loredana Lupu, Hendrik Rusche, International Mass Spectrometry Conference . 2018

机译：表位和亲和力测定结核分枝杆菌的重组分枝杆菌AG85B使用蛋白水解 - 亲和力质谱和生物传感器分析朝向抗AG85抗体的抗原
5. The identification and elimination of immunosuppressive Mycobacterium bovis BCG genes to create novel rBCG vectors that generate increased CD8+ T cell responses [D] . Panas, Michael William 2011

机译：鉴定和消除免疫抑制牛分枝杆菌BCG基因以创建产生增加的CD8 + T细胞反应的新型rBCG载体
6. Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG. [O] . L De Wit, A de la Cuvellerie, J Ooms, 2017

机译：牛分枝杆菌BCG的32 kDa蛋白质基因（抗原85 A）的核苷酸序列。
7. Nucleotide sequence of the 32 kDa-protein gene (antigen 85 A) of Mycobacterium bovis BCG. [O] . De Wit, L, de la Cuvellerie, A, Ooms, J, 1990

机译：牛分枝杆菌BCG的32 kDa蛋白质基因（抗原85 A）的核苷酸序列。

The genes coding for the antigen 85 complexes of Mycobacterium tuberculosis and Mycobacterium bovis BCG are members of a gene family: cloning sequence determination and genomic organization of the gene coding for antigen 85-C of M. tuberculosis.

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