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首页> 美国卫生研究院文献>Infection and Immunity >Comparative immunogenicity of conjugates composed of the Staphylococcus aureus type 8 capsular polysaccharide bound to carrier proteins by adipic acid dihydrazide or N-succinimidyl-3-(2-pyridyldithio)propionate.
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Comparative immunogenicity of conjugates composed of the Staphylococcus aureus type 8 capsular polysaccharide bound to carrier proteins by adipic acid dihydrazide or N-succinimidyl-3-(2-pyridyldithio)propionate.

机译：由己二酸二酰肼或N-琥珀酰亚胺基-3-（2-吡啶基二硫代）丙酸酯与载体蛋白结合的由金黄色葡萄球菌8型荚膜多糖组成的结合物的比较免疫原性。

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摘要

Staphylococcus aureus type 8 capsular polysaccharide (CP) was conjugated either to diphtheria toxoid or to Pseudomonas aeruginosa recombinant exoprotein A by using adipic acid dihydrazide (ADH) or N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) as the joining reagent. The polysaccharide/protein ratios of these two pairs of conjugates were similar. The two synthetic schemes bound the linker to the carboxyls of the type 8 CP by carbodiimide-mediated condensation. ADH was bound to the carboxyls of the protein, whereas SPDP reacted with the amino groups of the protein. Intermolecular linking of the carrier protein, caused by the carbodiimide during the conjugation reaction with the type 8 CP derivative, probably accounts for the larger size of the conjugates formed with ADH compared with those formed with SPDP. Both conjugates synthesized with ADH elicited higher levels of CP antibodies, especially after the first immunization, than did those prepared with SPDP. Similar levels of exoprotein A antibodies were elicited by both conjugates. Higher levels of diphtheria toxoid antibodies were elicited by the conjugate prepared with SPDP than by the one prepared with ADH. The basis for the differences in the immunogenicities of these two pairs of S. aureus type 8 CP conjugates is discussed.
机译：使用己二酸二酰肼（ADH）或N-琥珀酰亚胺基-3-（2-吡啶基二硫代）丙酸酯（SPDP）将金黄色葡萄球菌8型荚膜多糖（CP）与白喉类毒素或铜绿假单胞菌重组外蛋白A缀合。这两对结合物的多糖/蛋白质比率相似。两种合成方案通过碳二亚胺介导的缩合将连接子结合到8 CP型羧基上。 ADH与蛋白质的羧基结合，而SPDP与蛋白质的氨基反应。由碳二亚胺与8型CP衍生物的共轭反应引起的载体蛋白的分子间连接，可能与ADP形成的结合物相比，SPDP形成的结合物更大。与SPDP制备的结合物相比，用ADH合成的两种结合物均能引发更高水平的CP抗体，尤其是在首次免疫后。两种缀合物均引发相似水平的外蛋白A抗体。用SPDP制备的结合物比用ADH制备的结合物引起的白喉类毒素抗体水平更高。讨论了这两对金黄色葡萄球菌8型CP偶联物的免疫原性差异的基础。

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