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首页> 美国卫生研究院文献>Infection and Immunity >Phase variation of slime production in Staphylococcus aureus: implications in colonization and virulence.
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Phase variation of slime production in Staphylococcus aureus: implications in colonization and virulence.

机译：金黄色葡萄球菌的煤泥生产的相变：对定殖和毒力的影响。

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Two methods commonly used for slime detection in coagulase-negative staphylococci (tube biofilm formation and colony morphology in Congo red agar) were used to study 144 ruminant mastitis Staphylococcus aureus strains. Slime production was detected in 21 strains. A majority of cells (85%) in slime-producing (SP) strains and a minority of cells (5%) in non-slime-producing (NSP) strains showed a condensed exopolysaccharide matrix (slime) surrounding the bacterial cell wall, as revealed by electron microscopy and immunofluorescence. In vivo slime production was also detected immunohistochemically after experimental infection of the mammary gland in sheep. Upon repeated subcultures in Congo red agar, NSP variants were obtained from four ovine and four bovine SP strains at a frequency ranging from 0.5 x 10(-4) to 10(-4). Because SP variants could not be obtained from NSP strains within this range or at a higher frequency, they were obtained by the tube biofilm formation (requiring repeated subculturing of NSP strains in tryptic soy broth containing 2% glucose for subsequent recovery of colonies adherent to the walls of the culture tubes). In experimental challenge, the SP variant showed a significantly higher colonization capacity than did the NSP variant of the same strain used (P < 0.001). However, the NSP variant had a higher virulence than did the SP variant (P < 0.001). These results may help to explain the different roles of S. aureus slime production cell types (SP and NSP) coexisting in disease.
机译：两种常用的凝固酶阴性葡萄球菌粘液检测方法（刚果红琼脂中的试管生物膜形成和菌落形态）被用于研究144株反刍类乳腺炎金黄色葡萄球菌菌株。在21个菌株中检测到了粘液产生。粘液产生（SP）菌株中的大多数细胞（85％）和非粘液产生（NSP）菌株中的少数细胞（5％）在细菌细胞壁周围显示出浓缩的胞外多糖基质（粘液），通过电子显微镜和免疫荧光显示。在绵羊乳腺的实验性感染后，还通过免疫组织化学方法检测了体内的粘液产生。在刚果红琼脂中重复传代培养后，从四个绵羊和四个牛SP菌株中以0.5 x 10（-4）到10（-4）的频率获得NSP变异体。由于不能从该范围或更高频率的NSP菌株中获得SP变体，因此它们是通过管生物膜形成获得的（要求将NSP菌株在含2％葡萄糖的胰蛋白酶大豆肉汤中重复传代培养，以便随后回收附着在其上的菌落。培养管壁）。在实验挑战中，SP变体显示的定殖能力明显高于所用相同菌株的NSP变体（P <0.001）。但是，NSP变种比SP变种具有更高的毒力（P <0.001）。这些结果可能有助于解释疾病中共存的金黄色葡萄球菌粘液产生细胞类型（SP和NSP）的不同作用。

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