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首页> 美国卫生研究院文献>Infection and Immunity >Analysis of cytokine mRNA expression in Listeria-resistant C57BL/6 and Listeria-susceptible A/J mice during Listeria monocytogenes infection.
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Analysis of cytokine mRNA expression in Listeria-resistant C57BL/6 and Listeria-susceptible A/J mice during Listeria monocytogenes infection.

机译：在单核细胞增生李斯特菌感染过程中对李斯特菌耐药的C57BL / 6和易受李斯特菌感染的A / J小鼠的细胞因子mRNA表达进行分析。

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This laboratory previously reported that mRNA expression for many cytokines, as determined by reverse transcription-polymerase chain reaction analysis, is induced rapidly in the spleen during murine listeriosis. In the present study, the patterns of cytokine mRNA expression in spleens and livers of Listeria-resistant C57BL/6 and Listeria-susceptible A/J mice were compared. In addition, in situ hybridization was performed to evaluate the distributions of cytokine mRNA-expressing cells in these tissues. Listeria-resistant C57BL/6 mice demonstrated greater expression of gamma interferon (IFN-gamma) and granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNAs in the spleen than Listeria-susceptible A/J mice. Greater numbers of cells expressing IFN-gamma and GM-CSF mRNAs were observed by in situ hybridization in the spleens of C57BL/6 mice than in those of A/J mice. C57BL/6 and A/J mice did not differ in their expression of IFN-gamma mRNA in the liver. Nor did C57BL/6 and A/J mice differ in their expression of tumor necrosis factor alpha, interleukin-1 alpha (IL-1 alpha), IL-2, IL-4, or IL-6 mRNA in the liver or spleen, as determined by reverse transcription-polymerase chain reaction and in situ hybridization. These results indicate that the greater resistance of C57BL/6 mice to Listeria monocytogenes infection is associated with greater expression of IFN-gamma and GM-CSF mRNAs in the spleen and GM-CSF mRNA in the liver.
机译：该实验室先前报道，在鼠李斯特氏菌病期间，脾脏中迅速诱导了许多细胞因子的mRNA表达，这是通过逆转录聚合酶链反应分析确定的。在本研究中，比较了对李斯特菌耐药的C57BL / 6和对李斯特菌敏感的A / J小鼠的脾脏和肝脏中细胞因子mRNA的表达模式。另外，进行原位杂交以评估表达细胞因子mRNA的细胞在这些组织中的分布。与李斯特氏菌敏感的A / J小鼠相比，抵抗李斯特菌的C57BL / 6小鼠脾脏中的γ干扰素（IFN-γ）和粒细胞巨噬细胞集落刺激因子（GM-CSF）mRNA表达更高。通过原位杂交在C57BL / 6小鼠的脾脏中观察到比在A / J小鼠中更多的表达IFN-γ和GM-CSF mRNA的细胞。 C57BL / 6和A / J小鼠肝脏中IFN-γmRNA的表达没有差异。 C57BL / 6和A / J小鼠在肝脏或脾脏中的肿瘤坏死因子α，白介素-1α（IL-1 alpha），IL-2，IL-4或IL-6 mRNA的表达也没有差异，通过逆转录-聚合酶链反应和原位杂交确定。这些结果表明，C57BL / 6小鼠对单核细胞增生性李斯特菌感染的抵抗力更大，与脾脏中IFN-γ和GM-CSF mRNA的更高表达以及肝脏中GM-CSF mRNA的表达有关。

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