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Cloning and sequence analysis of the structural pilin gene of Brazilian purpuric fever-associated Haemophilus influenzae biogroup aegyptius.

机译:巴西紫癜热相关流感嗜血杆菌生物群埃及伊蚊的菌毛蛋白结构基因的克隆和序列分析。

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摘要

We have cloned and sequenced the Brazilian purpuric fever (BPF)-associated Haemophilus influenzae biogroup aegyptius (Hae) pilin gene. The sequence contained a 648-bp open reading frame encoding a mature pilin protein of 191 amino acids with a calculated mass of 20.5 kDa. There was 82% homology between the open reading frames of the BPF strain F3031 and H. influenzae type b (Hib) (strain M43) pilin genes and 71% homology at the amino acid level between the mature pilin proteins. However, areas of diversity were noted throughout the gene. A 17-bp probe corresponding to an area of diversity in the N-terminal region of the BPF-associated gene hybridized with other BPF strains but not with non-BPF Hae or Hib. In summary, the pilin protein of BPF-associated Hae is highly homologous to Hib pilin yet remains structurally distinct.
机译:我们已经克隆和测序与巴西紫癜热(BPF)相关的流感嗜血杆菌生物群埃及(Hae)菌毛蛋白基因。该序列包含一个648 bp的开放阅读框,该阅读框编码191个氨基酸的成熟菌毛蛋白,计算质量为20.5 kDa。 BPF菌株F3031与b型流感嗜血杆菌(Hib)(M43菌株)菌毛蛋白基因的开放阅读框之间有82%的同源性,成熟的菌毛蛋白之间的氨基酸水平有71%的同源性。但是,在整个基因中都注意到了多样性区域。一个17 bp的探针,对应于与其他BPF菌株杂交但不与非BPF Hae或Hib杂交的BPF相关基因N末端区域的多样性区域。总之,与BPF相关的Hae的pilin蛋白与Hib pilin高度同源,但在结构上仍然不同。

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