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Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay.

机译：从霍乱弧菌O26纯化的非膜破坏性细胞毒素（NMDCY）单克隆抗体的产生以及霍乱弧菌和其他肠道细菌菌株之间NMDCY的分布通过单克隆-多克隆三明治酶联免疫吸附法测定。

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The distribution of a newly described secretogenic non-membrane-damaging cytotoxin (NMDCY) among strains of Vibrio cholerae and other enteric bacteria was determined. To accomplish this, monoclonal antibodies against NMDCY were prepared and a sandwich monoclonal-polyclonal enzyme-linked immunosorbent assay (ELISA) was developed. By the sandwich ELISA, it was determined that 55.6% of the 412 strains of V. cholerae examined produced NMDCY at varying concentrations while 76, 37.9, and 15.6% of the clinical strains of Vibrio parahaemolyticus, Aeromonas spp., and Shigella spp., respectively, produced NMDCY. Because of its enterotoxigenic potential and based on its widespread distribution among strains of V. cholerae, we believe that NMDCY may constitute an important virulence determinant in the cascade of events which enable the organism to precipitate the disease.

机译：确定了霍乱弧菌和其他肠道细菌菌株中新描述的分泌性非膜破坏性细胞毒素（NMDCY）的分布。为此，制备了抗NMDCY的单克隆抗体，并开发了一种夹心单克隆-多克隆酶联免疫吸附测定（ELISA）。通过夹心ELISA，确定所检查的412株霍乱弧菌中有55.6％产生了不同浓度的NMDCY，而副溶血性弧菌，气单胞菌和志贺氏菌的临床菌株分别有76、37.9和15.6％。分别产生了NMDCY。由于其具有产毒潜力，并基于其在霍乱弧菌菌株之间的广泛分布，我们相信NMDCY可能构成一连串事件的重要毒力决定因素，这些事件使有机体能够促使疾病发作。

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期刊名称 Infection and Immunity
作者
P K Saha; G B Nair;
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年(卷),期 1997(65),2
年度 1997
页码 801–805
总页数 5
原文格式 PDF
正文语种
中图分类免疫学;病毒传染病;
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专利

1. Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay. [J] . P K Saha, G B Nair Infection and immunity . 1997,第2期

机译：从霍乱弧菌O26纯化的非膜破坏性细胞毒素（NMDCY）单克隆抗体的产生以及霍乱弧菌和其他肠道细菌菌株之间NMDCY的分布，通过单克隆-多克隆三明治酶联免疫吸附法测定。
2. Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay. [J] . P K Saha, G B Nair Infection and immunity . 1997,第2期

机译：从霍乱弧菌O26纯化的非膜破坏性细胞毒素（NMDCY）单克隆抗体的产生以及霍乱弧菌和其他肠道细菌菌株之间NMDCY的分布，通过单克隆-多克隆三明治酶联免疫吸附法测定。
3. Purification of Vibrio cholerae fur and estimation of its intracellular abundance by antibody sandwich enzyme-linked immunosorbent assay. [J] . P I Watnick, T Eto, H Takahashi, Journal of bacteriology . 1997,第1期

机译：霍乱弧菌的纯化和通过抗体夹心酶联免疫吸附法评估其胞内丰度。
4. Distribution of neuraminidase in vibrio cholerae serogroup O1 and O139 strains [C] . Yuanming Zhang, Biao Kan Guoming Qi, Yanqing Liu, 7th China-Japan International Congress of Microbiology Shanghai Symposium-2000 4-6 August 2000 Shanghai . 2000

机译：神经氨酸酶在霍乱弧菌血清群O1和O139中的分布
5. PRODUCTION AND EVALUATION OF ANTISERA AND MONOCLONAL ANTIBODIES DIRECTED AGAINST THE SEROVAR-SPECIFIC LIPOPOLYSACCHARIDE ANTIGENS OF VIBRIO CHOLERAE. [D] . ADAMS, LINDA BENTON. 1987

机译：针对霍乱弧菌的血清特异性脂多糖多糖抗原生产和评估的抗体和单克隆抗体。
6. Purification of Vibrio cholerae fur and estimation of its intracellular abundance by antibody sandwich enzyme-linked immunosorbent assay. [O] . P I Watnick, T Eto, H Takahashi, 1997

机译：霍乱弧菌的纯化和通过抗体夹心酶联免疫吸附法评估其细胞内丰度。
7. Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay [O] . P K Saha, G B Nair 1997

机译：从Vibrio Cholera O26纯化的非膜损伤细胞毒素（NMDcy）的单克隆抗体的生产和由单克隆 - 多克隆夹心酶联免疫吸附测定测定的振动慢性慢性菌株和其他肠溶细菌中NMDcy的分布

Production of monoclonal antibodies to the non-membrane-damaging cytotoxin (NMDCY) purified from Vibrio cholerae O26 and distribution of NMDCY among strains of Vibrio cholerae and other enteric bacteria determined by monoclonal-polyclonal sandwich enzyme-linked immunosorbent assay.

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