• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Infection and Immunity >Purified Lipopolysaccharide from Francisella tularensis Live Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon
【2h】

Purified Lipopolysaccharide from Francisella tularensis Live Vaccine Strain (LVS) Induces Protective Immunity against LVS Infection That Requires B Cells and Gamma Interferon

机译：来自土拉弗朗西斯菌活疫苗株（LVS）的纯化的脂多糖诱导抗需要B细胞和γ干扰素的LVS感染的保护性免疫。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Previous results have demonstrated that nonspecific protective immunity against lethal Francisella tularensis live vaccine strain (LVS) or Listeria monocytogenes infection can be stimulated either by sublethal infection with bacteria or by treatment with bacterial DNA given 3 days before lethal challenge. Here we characterize the ability of purified lipopolysaccharide (LPS) from F. tularensis LVS to stimulate similar early protective immunity. Treatment of mice with surprisingly small amounts of LVS LPS resulted in very strong and long-lived protection against lethal LVS challenge within 2 to 3 days. Despite this strong protective response, LPS purified from F. tularensis LVS did not activate murine B cells for proliferation or polyclonal immunoglobulin secretion, nor did it activate murine splenocytes for secretion of interleukin-4 (IL-4), IL-6, IL-12, or gamma interferon (IFN-γ). Immunization of mice with purified LVS LPS induced a weak specific anti-LPS immunoglobulin M (IgM) response and very little IgG; however, infection of mice with LVS bacteria resulted in vigorous IgM and IgG, particularly IgG2a, anti-LPS antibody responses. Studies using various immunodeficient mouse strains, including LPS-hyporesponsive C3H/HeJ mice, μMT− (B-cell-deficient) knockout mice, and IFN-γ-deficient mice, demonstrated that the mechanism of protection does not involve recognition through the Lpsn gene product; nonetheless, protection was dependent on B cells as well as IFN-γ.
机译：以前的结果表明，通过致死性感染细菌或在致死性攻击前三天给予细菌DNA处理，可以刺激针对致死性弗拉氏菌活疫苗株（LVS）或单核细胞增生李斯特菌感染的非特异性保护性免疫。在这里，我们表征了从杜氏烟草LVS纯化的脂多糖（LPS）刺激类似的早期保护性免疫的能力。用令人惊讶的少量LVS LPS治疗小鼠，可在2至3天内对致命的LVS攻击产生非常强大且长期的保护。尽管有这种强烈的保护性反应，但从土拉弗朗西斯（F. tularensis）LVS纯化的LPS并未激活鼠B细胞的增殖或多克隆免疫球蛋白分泌，也没有激活鼠脾细胞的白介素4（IL-4），IL-6，IL- 12或γ干扰素（IFN-γ）。用纯化的LVS LPS免疫小鼠可引起微弱的特异性抗LPS免疫球蛋白M（IgM）反应和极少的IgG。但是，用LVS细菌感染小鼠会导致强烈的IgM和IgG，特别是IgG2a抗LPS抗体反应。使用各种免疫缺陷小鼠品系的研究，包括LPS低反应性C3H / HeJ小鼠，μMT-（B细胞缺陷型）基因敲除小鼠和IFN-γ缺陷型小鼠，证明了保护机制确实不涉及通过Lps n 基因产物的识别；尽管如此，保护还是依赖于B细胞以及IFN-γ。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号