• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Infection and Immunity >Effector CD8+ T Cells Are Generated in Response to an Immunodominant Epitope in Type III Effector YopE during Primary Yersinia pseudotuberculosis Infection
【2h】

Effector CD8+ T Cells Are Generated in Response to an Immunodominant Epitope in Type III Effector YopE during Primary Yersinia pseudotuberculosis Infection

机译：在原发性耶尔森氏菌假结核感染期间对III型效应物YopE中的免疫抗原决定簇产生反应产生效应物CD8 + T细胞。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

YopE is a virulence factor that is secreted into host cells infected by Yersinia species. The YopE C-terminal domain has GTPase-activating protein (GAP) activity. The YopE N-terminal domain contains an epitope that is an immunodominant CD8+ T cell antigen during primary infection of C57BL/6 mice with Yersinia pseudotuberculosis. The characteristics of the CD8+ T cells generated in response to the epitope, which comprises YopE amino acid residues 69 to 77 (YopE69–77), and the features of YopE that are important for antigenicity during primary infection, are unknown. Following intravenous infection of naïve C57BL/6 mice with a yopE GAP mutant (the R144A mutant), flow cytometry analysis of splenocytes by tetramer and intracellular cytokine staining over a time course showed that YopE69–77-specific CD8+ T cells producing gamma interferon (IFN-γ) and tumor necrosis factor alpha (TNF-α) were generated by day 7, with a peak at day 14. In addition, ∼80% of YopE69–77-specific CD8+ T cells were positive for KLRG1, a memory phenotype marker, at day 21. To determine if residues that regulate YopE activity by ubiquitination or membrane localization affect the antigenicity of YopE69–77, mice were infected with a yopE ubiquitination or membrane localization mutant (the R62K or L55N I59N L63N mutant, respectively). These mutants elicited YopE69–77-specific CD8+ T cells producing IFN-γ and TNF-α with kinetics and magnitudes similar to those of the parental R144A strain, indicating that primary infection primes effector CD8+ T cells independently of the ubiquitination or membrane localization of YopE. Additionally, at day 7, there was an unexpected positive correlation between the numbers of YopE69–77-specific CD8+ T cells and CD11b+ cells, but not between the numbers of YopE69–77-specific CD8+ T cells and bacterial cells, in spleens, suggesting that the innate immune response contributes to the immunodominance of YopE69–77.
机译：YopE是一种毒力因子，被分泌到被耶尔森菌感染的宿主细胞中。 YopE C末端域具有GTPase激活蛋白（GAP）活性。 YopE N末端结构域包含一个抗原决定簇，该抗原决定簇是C57BL / 6小鼠初次感染耶尔森氏菌假性结核病期间的一种免疫性CD8 + T细胞抗原。响应表位而产生的CD8 + T细胞的特征，其包含YopE氨基酸残基69至77（YopE69–77），以及对于初次感染时的抗原性很重要的YopE特征，是未知的。在使用yopE GAP突变体（R144A突变体）对纯净的C57BL / 6小鼠进行静脉感染后，通过四聚体和细胞内细胞因子染色对脾细胞进行流式细胞术分析显示，YopE69–77特异性CD8 + 在第7天产生了产生γ干扰素（IFN-γ）和肿瘤坏死因子α（TNF-α）的T细胞，并在第14天达到峰值。此外，约80％的YopE69-77特异性CD8 + T细胞在第21天对记忆表型标记KLRG1呈阳性。为了确定通过泛素化或膜定位来调节YopE活性的残基是否影响YopE69-77的抗原性，将小鼠感染了yopE泛素化或膜定位突变体（分别为R62K或L55N I59N L63N突变体）。这些突变体引发了YopE69–77特异性CD8 + T细胞，产生的IFN-γ和TNF-α的动力学和大小与亲代R144A菌株相似，表明原发感染引发效应子CD8 + T细胞独立于YopE的泛素化或膜定位。此外，在第7天，YopE69–77特异性CD8 + T细胞和CD11b + 细胞之间存在出乎意料的正相关，而与YopE69-77特异性脾脏CD8 + T细胞和细菌细胞，提示先天免疫应答有助于YopE69-77的免疫优势。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号