• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Materials >A Direct Electric Field-Aided Biomimetic Mineralization System for Inducing the Remineralization of Dentin Collagen Matrix
【2h】

A Direct Electric Field-Aided Biomimetic Mineralization System for Inducing the Remineralization of Dentin Collagen Matrix

机译：直接电场辅助仿生矿化系统诱导牙本质胶原基质的再矿化

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

This in vitro study aimed to accelerate the remineralization of a completely demineralized dentine collagen block in order to regenerate the dentinal microstructure of calcified collagen fibrils by a novel electric field-aided biomimetic mineralization system in the absence of non-collagenous proteins. Completely demineralized human dentine slices were prepared using ethylene diamine tetraacetic acid (EDTA) and treated with guanidine hydrochloride to extract the bound non-collagenous proteins. The completely demineralized dentine collagen blocks were then remineralized in a calcium chloride agarose hydrogel and a sodium hydrogen phosphate and fluoride agarose hydrogel. This process was accelerated by subjecting the hydrogels to electrophoresis at 20 mA for 4 and 12 h. X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM) were used to evaluate the resultant calcification of the dentin collagen matrix. SEM indicated that mineral particles were precipitated on the intertubular dentin collagen matrix; these densely packed crystals mimicked the structure of the original mineralized dentin. However, the dentinal tubules were not occluded by the mineral crystals. XRD and EDX both confirmed that the deposited crystals were fluorinated hydroxyapatite. TEM revealed the existence of intrafibrillar and interfibrillar mineralization of the collagen fibrils. A novel electric field-aided biomimetic mineralization system was successfully developed to remineralize a completely demineralized dentine collagen matrix in the absence of non-collagenous proteins. This study developed an accelerated biomimetic mineralization system which can be a potential protocol for the biomineralization of dentinal defects.
机译：这项体外研究旨在加速完全脱矿质的牙本质胶原蛋白块的再矿化，以便在不存在非胶原蛋白的情况下通过新型电场辅助仿生矿化系统再生钙化胶原蛋白原纤维的牙本质组织。使用乙二胺四乙酸（EDTA）制备完全脱矿质的人牙本质切片，并用盐酸胍处理以提取结合的非胶原蛋白。然后将完全脱盐的牙本质胶原蛋白块在氯化钙琼脂糖水凝胶和磷酸氢钠和氟化物琼脂糖水凝胶中再矿化。通过在20 mA下对水凝胶进行电泳4和12 h来加速此过程。 X射线衍射（XRD），扫描电子显微镜（SEM），能量色散X射线光谱（EDX）和透射电子显微镜（TEM）用于评估牙本质胶原蛋白基质的钙化程度。扫描电镜显示矿物质颗粒沉淀在肾小管间牙本质胶原基质上。这些密集堆积的晶体模仿了原始矿化牙本质的结构。但是，牙本质小管并没有被矿物晶体阻塞。 XRD和EDX均证实沉积的晶体是氟化羟基磷灰石。 TEM显示胶原原纤维的原纤维内和原纤维间矿化。成功开发了一种新型的电场辅助仿生矿化系统，可在不存在非胶原蛋白的情况下对完全脱矿的牙本质胶原蛋白基质进行再矿化。这项研究开发了一种加速的仿生矿化系统，该系统可以作为牙本质缺损生物矿化的潜在方案。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号