• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Immunology >Cloned mouse mast cells derived from immunized lymph node cells and from foetal liver cells exhibit characteristics of bone marrow-derived mast cells containing chondroitin sulphate E proteoglycan.
【2h】

Cloned mouse mast cells derived from immunized lymph node cells and from foetal liver cells exhibit characteristics of bone marrow-derived mast cells containing chondroitin sulphate E proteoglycan.

机译：源自免疫淋巴结细胞和胎儿肝细胞的克隆小鼠肥大细胞表现出含有硫酸软骨素E蛋白多糖的骨髓源肥大细胞的特征。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cloned mouse mast cells which were T cell growth-dependent were derived both from immunized lymph node and from foetal liver, and were found to be morphologically and biochemically similar to mast cells previously differentiated in vitro from mouse bone marrow (BMMC). These two T cell growth-dependent mouse mast cell clones were identical to the BMMC in their preferential synthesis of chondroitin sulphate E proteoglycan rather than heparin proteoglycan. The hydrodynamic size of the cell-associated proteoglycan from each of the three mast cell sources was 150,000-250,000 mol. wt.; and that of the covalently bound glycosaminoglycans was 13,000-25,000 mol. wt. Chondroitinase ABC digestion of the [35S]proteoglycans from both cloned mast cells, as well as the BMMC, yielded only two disaccharides which comigrated on ascending thin layer chromatography with delta Di-4S and delta Di-diSE standards, respectively. Quantification of the radioactivity in the enzyme digests revealed that one-sixth to one-half of the resulting disaccharides were disulphated, similar to that found in BMMC containing chondroitin sulphate E. When sensitized with monoclonal IgE, washed, and subsequently challenged with specific antigen, each of the two cloned mast cells generated more than 100 ng of leukotriene C4 (LTC4)/10(6) cells, but only 3-12 ng leukotriene B4 (LTB4)/10(6) cells, characteristics also observed for the BMMC. Based upon these observations, it is concluded that the cloned mast cells from lymph node and liver and the bone marrow-derived mast cell belong to a distinct subclass of mast cells. These mast cells have been designated E-mast cells (E-MC) in order to distinguish them from heparin-containing mast cells (H-MC).
机译：T细胞生长依赖性的克隆的小鼠肥大细胞既来源于免疫淋巴结，又来源于胎儿肝脏，并且在形态和生化上类似于先前从小鼠骨髓（BMMC）体外分化出来的肥大细胞。这两个依赖T细胞生长的小鼠肥大细胞克隆在优先合成硫酸软骨素E蛋白聚糖而不是肝素蛋白聚糖方面与BMMC相同。来自三个肥大细胞来源的每一个的细胞相关蛋白聚糖的流体力学大小为150,000-250,000 mol。重量共价结合的糖胺聚糖的分子量为13,000-25,000mol。重量从两个克隆的肥大细胞以及BMMC的[35S]蛋白聚糖的软骨素酶ABC消化中，仅产生了两个二糖，分别在增量薄层色谱中分别用delta Di-4S和delta Di-diSE标准品进行了分离。酶消化物中放射性的定量分析表明，所得二糖的六分之一至二分之一被分解，类似于含有硫酸软骨素E的BMMC中的二糖。当用单克隆IgE敏化，洗涤并随后用特定抗原攻击时，两个克隆的肥大细胞中的每一个都产生了超过100 ng的白三烯C4（LTC4）/ 10（6）细胞，但仅产生了3-12 ng的白三烯B4（LTB4）/ 10（6）细胞，BMMC还具有这种特征。基于这些观察，可以得出结论，从淋巴结和肝脏克隆的肥大细胞以及骨髓来源的肥大细胞属于肥大细胞的独特亚类。这些肥大细胞被称为E肥大细胞（E-MC），以使其与含肝素的肥大细胞（H-MC）区别开来。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号