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首页> 美国卫生研究院文献>Immunology >The monoclonal antibody UCHL1 recognizes a 180000 MW component of the human leucocyte-common antigen CD45.
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The monoclonal antibody UCHL1 recognizes a 180000 MW component of the human leucocyte-common antigen CD45.

机译：单克隆抗体UCHL1识别人白细胞常见抗原CD45的180000 MW成分。

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摘要

The leucocyte-common antigen (L-CA or CD45) is a family of high molecular weight glycoproteins, ranging from 180,000 to 220,000 MW that are expressed only on cells of lymphoid and myeloid origin. CD45 monoclonal antibodies (mAbs) recognize epitopes present on all polypeptides of the family, while other mAbs, termed CD45R, recognize determinants found only on the 220,000 MW and 200,000 MW polypeptides. In contrast the mAb UCHL1 recognizes a 180,000 MW antigen. UCHL1-coupled Sepharose beads were used to absorb antigen from lysates of cell lines. CD45 mAbs bound to this immobilized antigen. Antigen immobilized with CD45 mAb-coupled Sepharose beads bound UCHL1. Antigen purified by absorption and elution from the MOLT-4 cell line with CD45 mAb-coupled beads yielded molecules of 180,000 and 190,000 MW. Reprecipitation of the eluted antigen with UCHL1 resulted in a 180,000 MW band only. In a reciprocal experiment, CD45 mAb reprecipitated a 180,000 MW molecule from purified UCHL1 antigen. UCHL1 and the CD45R mAb 2H4 showed a mutually exclusive pattern of reactivity with human T- and B-cell lines, but co-expression of the antigens was seen on two myeloid and one erythroleukaemic cell line. In contrast, epitopes recognized by other putative CD45R mAbs were co-expressed with UCHL1 both on myeloid, erythroid and many T- and B-cell lines. We conclude that UCHL1 recognizes an epitope present only on the 180,000 MW polypeptide of CD45. Expression of this antigen is essentially reciprocal to the epitope detected by the CD45R mAb 2H4.
机译：白细胞常见抗原（L-CA或CD45）是一类高分子量糖蛋白，范围从180,000到220,000 MW，仅在淋巴和髓样来源的细胞上表达。 CD45单克隆抗体（mAb）识别该家族所有多肽上存在的表位，而其他称为CD45R的mAb则仅识别在220,000 MW和200,000 MW多肽上发现的决定簇。相反，mAb UCHL1识别180,000 MW抗原。 UCHL1偶联的琼脂糖珠用于吸收细胞系裂解物中的抗原。 CD45 mAb与该固定抗原结合。用CD45 mAb偶联的琼脂糖珠固定的抗原结合了UCHL1。通过用CD45 mAb偶联的珠子从MOLT-4细胞系吸收和洗脱纯化的抗原产生180,000和190,000 MW的分子。用UCHL1对洗脱的抗原进行再沉淀仅产生了180,000 MW条带。在对等实验中，CD45 mAb从纯化的UCHL1抗原中再沉淀出180000 MW分子。 UCHL1和CD45R mAb 2H4显示出与人类T细胞和B细胞系相互作用的互斥模式，但在两种髓样细胞和一种红白血病细胞系中发现了抗原的共表达。相反，被其他推定的CD45R mAb识别的表位与UCHL1在髓样，红系以及许多T细胞和B细胞系中共表达。我们得出的结论是，UCHL1识别仅在CD45的180,000 MW多肽上存在的表位。该抗原的表达基本上与CD45R mAb 2H4检测到的表位相对应。

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