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首页> 美国卫生研究院文献>Immunology >IL-10 augments CD23 expression on U937 cells and down-regulates IL-4-driven CD23 expression on cultured human blood monocytes: effects of IL-10 and other cytokines on cell phenotype and phagocytosis.
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IL-10 augments CD23 expression on U937 cells and down-regulates IL-4-driven CD23 expression on cultured human blood monocytes: effects of IL-10 and other cytokines on cell phenotype and phagocytosis.

机译：IL-10增强U937细胞上的CD23表达并下调培养的人血单核细胞上IL-4驱动的CD23表达：IL-10和其他细胞因子对细胞表型和吞噬作用的影响。

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The effects of human recombinant interleukin-10 (IL-14) on the expression of several markers on U937 and human peripheral blood monocytes was studied by immunofluorescence and fluorescence-activated cell sorter (FACS) analysis. IL-10 augmented Fc IgE receptor (Fc epsilon RII/CD23) further enhanced by cotreatment with IL-4 or interferon-gamma (IFN-gamma). In contrast, the basal level of Fc epsilon RII expression on blood monocytes appeared to fall in response to IL-10, and this effect became more evident on IL-4-treated cells. Furthermore, the constitutive and IFN-gamma-triggered Fc gamma RI/CD64 expression was augmented on both monocytes and U937 cells. Thus the expression of Fc gamma RII/CD32, Fc gamma/RIII/CD16, Fc alpha R/CD89, the receptor for complement components (CR1/CD35, CD3/CD11b, CR4/CD11c) and the receptor for transferrin/CD71 was not significantly influenced on IL-10-treated cells. IL-10 modestly triggered CD14 antigen expression on monocytes but not U937. The expression of intercellular adhesion molecule-1 (ICAM-1)/CD54 on monocytes was significantly inhibited by IL-10. As expected, a marked reduction of the constitutive as well as of the IFN-gamma or IL-4-driven expression on HLA-DR, HLA-DP and HLA-DQ was observed on IL-10-cultured monocytes. On the other hand, the expression of major histocompatibility complex (MHC) class I molecules was slightly and dose-dependently induced on IL-10-treated monocytes. The ability of blood monocytes to phagocytose IgG-sensitized ox erythrocytes, and to bind and ingest opsonized Escherichia coli or latex particles, was amplified by IL-10. Our data demonstrate that IL-10 modulates the expression of a wide variety of structures on human mononuclear phagocytes, and augments their phagocytic capacity.
机译：通过免疫荧光和荧光激活细胞分选仪（FACS）分析研究了人类重组白介素10（IL-14）对U937和人类外周血单核细胞上几种标志物表达的影响。通过与IL-4或干扰素-γ（IFN-γ）共同处理，IL-10增强的Fc IgE受体（Fc epsilon RII / CD23）进一步增强。相反，血液单核细胞上的FcεRII表达的基础水平似乎响应于IL-10而下降，并且这种作用在经IL-4处理的细胞上变得更加明显。此外，在单核细胞和U937细胞上，本构性和IFN-γ触发的FcγRI / CD64表达均增加。因此，FcγRII/ CD32，Fcγ/ RIII / CD16，FcαR/ CD89，补体成分的受体（CR1 / CD35，CD3 / CD11b，CR4 / CD11c）和转铁蛋白/ CD71的受体的表达不是。对经IL-10处理的细胞有显着影响。 IL-10适度触发了单核细胞上CD14抗原的表达，但未触发U937。 IL-10明显抑制单核细胞上的细胞间粘附分子-1（ICAM-1）/ CD54的表达。如所预期的，在IL-10培养的单核细胞上观察到HLA-DR，HLA-DP和HLA-DQ上的组成型以及IFN-γ或IL-4驱动的表达的显着降低。另一方面，主要组织相容性复合体（MHC）I类分子的表达在经IL-10处理的单核细胞上略有剂量依赖性。 IL-10增强了血液单核细胞吞噬IgG敏感的红血球以及结合和摄取调理过的大肠杆菌或乳胶颗粒的能力。我们的数据表明，IL-10调节人单核吞噬细胞上多种结构的表达，并增强其吞噬能力。

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