• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Immunology >Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those of human embryonic stem cell lines
【2h】

Immune-privileged embryonic Swiss mouse STO and STO cell-derived progenitor cells: major histocompatibility complex and cell differentiation antigen expression patterns resemble those of human embryonic stem cell lines

机译：免疫特权的胚胎瑞士小鼠STO和STO细胞来源的祖细胞：主要的组织相容性复合物和细胞分化抗原的表达模式与人胚胎干细胞系相似

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Embryonic mouse STO (S, SIM; T, 6-thioguanine resistant; O, ouabain resistant) and 3(8)21-enhanced green fluorescent protein (EGFP) cell lines exhibit long-term survival and hepatic progenitor cell behaviour after xenogeneic engraftment in non-immunosuppressed inbred rats, and were previously designated major histocompatibility complex (MHC) class I- and class II-negative lines. To determine the molecular basis for undetectable MHC determinants, the expression and haplotype of H-2K, H-2D, H-2L and I-A proteins were reassessed by reverse transcriptase–polymerase chain reaction (RT-PCR), cDNA sequencing, RNA hybridization, immunoblotting, quantitative RT-PCR (QPCR), immunocytochemistry and flow cytometry. To detect cell differentiation (CD) surface antigens characteristic of stem cells, apoptotic regulation or adaptive immunity that might facilitate progenitor cell status or immune privilege, flow cytometry was also used to screen untreated and cytokine [interferon (IFN)-γ]-treated cultures. Despite prior PCR genotyping analyses suggestive of H-2q haplotypes in STO, 3(8)21-EGFP and parental 3(8)21 cells, all three lines expressed H-2K cDNA sequences identical to those of d-haplotype BALB/c mice, as well as constitutive and cytokine-inducible H-2Kd determinants. In contrast, apart from H-2Ld[LOW] display in 3(8)21 cells, H-2Dd, H-2Ld and I-Ad determinants were undetectable. All three lines expressed constitutive and cytokine-inducible CD34; however, except for inducible CD117[LOW] expression in 3(8)21 cells, no expression of CD45, CD117, CD62L, CD80, CD86, CD90·1 or CD95L/CD178 was observed. Constitutive and cytokine-inducible CD95[LOW] expression was detected in STO and 3(8)21 cells, but not in 3(8)21-EGFP cells. MHC (class I+[LOW]/class II−) and CD (CD34+/CD80−/CD86−/CD95L−) expression patterns in STO and STO cell-derived progenitor cells resemble patterns reported for human embryonic stem cell lines. Whether these patterns reflect associations with mechanisms that are regulatory of immune privilege or functional tissue-specific plasticity is unknown.
机译：胚胎小鼠STO（S，SIM; T，6-硫鸟嘌呤抗性; O，哇巴因抗性）和3（8）21增强的绿色荧光蛋白（EGFP）细胞系在异种移植后表现出长期存活和肝祖细胞行为非免疫抑制的近交大鼠，先前被指定为主要组织相容性复合体（MHC）I类和II类阴性品系。为了确定无法检测到的MHC决定簇的分子基础，我们通过逆转录聚合酶链反应（RT-PCR），cDNA测序，RNA杂交对H-2K，H-2D，H-2L和IA蛋白的表达和单倍型进行了重新评估，免疫印迹，定量RT-PCR（QPCR），免疫细胞化学和流式细胞仪。为了检测干细胞的特征性细胞分化（CD）表面抗原，可能有助于祖细胞状态或免疫特权的凋亡调节或适应性免疫，还使用了流式细胞术来筛选未经处理和经细胞因子[干扰素（IFN）-γ]处理的培养物。尽管先前的PCR基因分型分析提示在STO，3（8）21-EGFP和亲本3（8）21细胞中存在H-2 q 单倍型，但所有三株系均表达与H-2K cDNA序列相同的H-2K cDNA序列。 d-单倍型BALB / c小鼠的模型，以及组成型和细胞因子诱导的H-2K d 决定簇。相反，除了在3（8）21个单元格中显示H-2L d [LOW] 之外，H-2D d ，H-2L d 和IA d 决定因素无法检测到。所有三个系均表达组成型和细胞因子可诱导的CD34。然而，除了在3（8）21细胞中诱导CD117 [LOW] 表达外，未观察到CD45，CD117，CD62L，CD80，CD86，CD90·1或CD95L / CD178的表达。在STO和3（8）21细胞中检测到组成型和细胞因子诱导的CD95 [LOW] 表达，但在3（8）21-EGFP细胞中未检测到。 MHC（I类 + [LOW] / II类-）和CD（CD34 + / CD80 - / STO和STO细胞来源的祖细胞中的CD86 - / CD95L -）表达模式类似于人类胚胎干细胞系报道的模式。这些模式是否反映了与调节免疫特权或功能性组织特异性可塑性的机制的关联尚不清楚。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号