首页> 美国卫生研究院文献>Indian Journal of Microbiology >Endophytic Ability of Different Isolates of Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin in Stem and Leaf Tissues of Maize (Zea mays L.)
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Endophytic Ability of Different Isolates of Entomopathogenic Fungi Beauveria bassiana (Balsamo) Vuillemin in Stem and Leaf Tissues of Maize (Zea mays L.)

机译:玉米(Zea mays L.)茎和叶组织中不同病原真菌球孢白僵菌(Balsamo)Vuillemin分离株的内生能力。

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摘要

The present study was conducted to examine the ability of six promising indigenous isolates of Beauveria bassiana (NBAII-Bb-5a, 7, 14, 19, 23 and 45) as an endophyte in maize stem and leaf tissues. Maize seedlings (var. Nithyashree) were inoculated with conidial suspensions and were examined for endophytic establishment in leaf and stems at different intervals during 15–90 days after treatment. All six isolates showed colonization in stem and leaf tissues with varying abilities of colonization and persistence. The mean percent colonization ranged from 7.41 to 20.37 % in older stem tissues and 3.70 to 21.29 % in young stem tissues and in leaf, it ranged from 6.46 to 27.78 % in older leaf tissues and 11.11 to 26.85 % in young leaf tissues. Among six isolates tested, Bb-23 isolate recorded the maximum mean colonization in older stem (20.37 %), older leaf (27.78 %) and in young stem (21.29 %). Bb-5a isolate showed maximum mean colonization in young leaf tissues (26.85 %). Persistence of inoculated fungal isolates decreased with increase in age of the plant. No physical symptoms of damage were observed in any of the B. bassiana treated plants. No colonization of B. bassiana was observed in the untreated control maize plants. The results obtained in plating and PCR techniques were similar with regard to the confirmation of endophytic establishment of B. bassiana. This study indicated the possibility of using B. bassiana as an endophyte in maize for management of maize stem borer, Chilo partellus.
机译:本研究旨在检查球孢白僵菌的六个有希望的本土分离株(NBAII-Bb-5a,7、14、19、23和45)作为玉米茎和叶组织中的内生菌的能力。用分生孢子悬浮液接种玉米幼苗(变种Nithyashree),并在处理后15-90天内以不同的间隔检查玉米叶片和茎中的内生植物。所有六个分离株均在茎和叶组织中定植,并具有不同的定植和持久能力。在较老的茎组织中,平均定植百分比范围为7.41%至20.37%,在较年轻的茎组织和叶片中,平均定殖率为3.70至21.29%,对于较老的叶子组织,其定殖范围为6.46至27.78%,对于较年轻的叶子组织,其定殖范围为11.11至26.85%。在测试的六个分离株中,Bb-23分离株在老茎(20.37%),老叶(27.78%)和年轻茎(21.29%)中记录了最大的平均定植。 Bb-5a分离株显示出在年轻叶片组织中的最大平均定植率(26.85%)。接种真菌分离株的持久性随植物年龄的增加而降低。在经球孢杆菌处理的任何植物中均未观察到损害的物理症状。在未处理的对照玉米植物中未观察到球孢杆菌的定植。在平板和PCR技术中获得的结果与确认球孢白僵菌的内生建立相似。这项研究表明在玉米中使用球芽孢杆菌作为内生菌来管理玉​​米stem,Chilo partellus的可能性。

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