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首页> 美国卫生研究院文献>Horticulture Research >Multiple quantitative trait loci contribute to resistance to bacterial canker incited by Pseudomonas syringae pv. actinidiae in kiwifruit (Actinidia chinensis)
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Multiple quantitative trait loci contribute to resistance to bacterial canker incited by Pseudomonas syringae pv. actinidiae in kiwifruit (Actinidia chinensis)

机译：多个数量性状基因座有助于抵抗丁香假单胞菌PV诱导的细菌性溃疡病。猕猴桃猕猴桃中的猕猴桃

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摘要

Pseudomonas syringae pv. actinidiae (Psa) biovar 3, a virulent, canker-inducing pathogen is an economic threat to the kiwifruit (Actinidia spp.) industry worldwide. The commercially grown diploid (2×) A. chinensis var. chinensis is more susceptible to Psa than tetraploid and hexaploid kiwifruit. However information on the genetic loci modulating Psa resistance in kiwifruit is not available. Here we report mapping of quantitative trait loci (QTLs) regulating resistance to Psa in a diploid kiwifruit population, derived from a cross between an elite Psa-susceptible ‘Hort16A’ and a resistant male breeding parent P1. Using high-density genetic maps and intensive phenotyping, we identified a single QTL for Psa resistance on Linkage Group (LG) 27 of ‘Hort16A’ revealing 16–19% phenotypic variance and candidate alleles for susceptibility and resistance at this loci. In addition, six minor QTLs were identified in P1 on distinct LGs, exerting 4–9% variance. Resistance in the F1 population is improved by additive effects from ‘Hort16A’ and P1 QTLs providing evidence that divergent genetic pathways interact to combat the virulent Psa strain. Two different bioassays further identified new QTLs for tissue-specific responses to Psa. The genetic marker at LG27 QTL was further verified for association with Psa resistance in diploid Actinidia chinensis populations. Transcriptome analysis of Psa-resistant and susceptible genotypes in field revealed hallmarks of basal defense and provided candidate RNA-biomarkers for screening for Psa resistance in greenhouse conditions.
机译：丁香假单胞菌PV。猕猴桃（Psa）biovar 3是一种致病性极强的致病性致病菌，对全球的猕猴桃（Actinidia spp。）工业构成经济威胁。商业种植的二倍体（2x）中华曲霉中华猕猴桃比四倍体和六倍体猕猴桃更易受Psa感染。但是，尚无有关调节奇异果中Psa抗性的遗传基因座的信息。在这里，我们报告了定量性状基因座（QTL）的定位图，该性状位点调节了二倍体猕猴桃种群对Psa的抗性，这是由Psa敏感的'Hort16A'优良品种与抗性雄性育种亲本P1之间的杂交得到的。使用高密度遗传图谱和深入的表型分析，我们在“ Hort16A”的连锁组（LG）27上鉴定了一个单一的Psa抗性QTL，揭示了16-19％的表型变异以及该基因座的易感性和抗性候选等位基因。此外，在不同LG的P1中鉴定出6个次要QTL，差异为4-9％。 “ Hort16A”和P1 QTL的加性效应改善了F1种群的抗药性，提供了不同的遗传途径相互作用以对抗强毒的Psa菌株的证据。两种不同的生物测定进一步确定了针对Psa的组织特异性反应的新QTL。进一步验证了LG27 QTL的遗传标记与二倍体猕猴桃种群中的Psa抗性相关。田间对Psa抗性和易感基因型的转录组分析揭示了基础防御的标志，并为温室条件下的Psa抗性筛选提供了候选RNA生物标记。

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