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Genomic collinearity and the genetic architecture of floral differences between the homoploid hybrid species Iris nelsonii and one of its progenitors Iris hexagona

机译:单倍体杂种鸢尾鸢尾和其祖先之一鸢尾鸢尾之间的基因组共线性和花艺差异的遗传结构

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摘要

Hybrid speciation represents a relatively rapid form of diversification. Early models of homoploid hybrid speciation suggested that reproductive isolation between the hybrid species and progenitors primarily resulted from karyotypic differences between the species. However, genic incompatibilities and ecological divergence may also be responsible for isolation. Iris nelsonii is an example of a homoploid hybrid species that is likely isolated from its progenitors primarily by strong prezygotic isolation, including habitat divergence, floral isolation and post-pollination prezygotic barriers. Here, we used linkage mapping and quantitative trait locus (QTL) mapping approaches to investigate genomic collinearity and the genetic architecture of floral differences between I. nelsonii and one of its progenitor species I. hexagona. The linkage map produced from this cross is highly collinear with another linkage map produced between I. fulva and I. brevicaulis (the two other species shown to have contributed to the genomic makeup of I. nelsonii), suggesting that karyotypic differences do not contribute substantially to isolation in this homoploid hybrid species. Similar to other studies of the genetic architecture of floral characteristics, at least one QTL was found that explained >20% variance in each color trait, while minor QTLs were detected for each morphological trait. These QTLs will serve as hypotheses for regions under selection by pollinators.
机译:杂交物种代表了相对快速的多样化形式。早期的同倍体杂种形成模型表明杂种与祖先之间的生殖隔离主要是由于种间的核型差异所致。但是,基因的不相容性和生态差异也可能是造成隔离的原因。鸢尾鸢尾(Iris nelsonii)是同倍体杂种的一个例子,很可能主要通过强合子前分离来从其祖细胞中分离出来,这些合子包括生境散布,花分离和授粉后合子前的障碍。在这里,我们使用连锁作图和数量性状基因座(QTL)作图方法来调查纳尔逊伊尔森氏菌与其祖先物种之一六边形之间的基因组共线性和花艺差异的遗传结构。从该杂交产生的连锁图与富叶艾弗氏菌和短尾梭毛虫之间的另一连锁图高度共线(这两个另外的物种被证明对纳尔逊纳希菌的基因组构成有贡献),表明核型差异基本上没有贡献分离该同倍体杂种。与其他有关花卉特征遗传结构的研究相似,发现至少一个QTL解释了每种颜色性状的变异> 20%,而每种形态性状均检测到较小的QTL。这些QTL将作为传粉媒介选择区域的假设。

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