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首页> 美国卫生研究院文献>Heredity >A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm
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A FISH-based chromosome map for the European corn borer yields insights into ancient chromosomal fusions in the silkworm

机译：基于FISH的欧洲玉米bore的染色体图可深入了解家蚕中的古代染色体融合体

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摘要

A significant feature of the genomes of Lepidoptera, butterflies and moths, is the high conservation of chromosome organization. Recent remarkable progress in genome sequencing of Lepidoptera has revealed that syntenic gene order is extensively conserved across phylogenetically distant species. The ancestral karyotype of Lepidoptera is thought to be n=31; however, that of the most well-studied moth, Bombyx mori, is n=28, and diverse studies suggest that three chromosomal fusion events occurred in this lineage. To identify the boundaries between predicted ancient fusions involving B. mori chromosomes 11, 23 and 24, we constructed fluorescence in situ hybridization (FISH)-based chromosome maps of the European corn borer, Ostrinia nubilalis (n=31). We first determined a 511 Mb genomic sequence of the Asian corn borer, O. furnacalis, a congener of O. nubilalis, and isolated bacterial artificial chromosomes and fosmid clones that were expected to localize in candidate regions for the boundaries using these sequences. Combined with FISH and genetic analysis, we narrowed down the candidate regions to 40 kb–1.5 Mb, in strong agreement with a previous estimate based on the genome of a butterfly, Melitaea cinxia. The significant difference in the lengths of the candidate regions where no functional genes were observed may reflect the evolutionary time after fusion events.
机译：鳞翅目，蝴蝶和飞蛾的基因组的重要特征是染色体组织的高度保守性。鳞翅目的基因组测序最近的显着进展表明，同系基因顺序在整个系统发育远缘物种中都得到了广泛的保守。鳞翅目的祖先核型被认为是n = 31。但是，研究最深入的蛾（家蚕）的n = 28，并且各种研究表明，该谱系中发生了3个染色体融合事件。为了确定涉及桑蚕芽孢杆菌第11、23和24号染色体的预计古代融合之间的边界，我们构建了基于荧光原位杂交（FISH）的欧洲玉米bore，玉米rin（Nostrinia nubilalis，n = 31）的染色体图。我们首先确定了亚洲玉米rO。furnacalis（一种O. nubilalis的同种动物）的511 Mb基因组序列，并分离出了人工细菌染色体和fosmid克隆，使用这些序列可以定位在边界的候选区域中。结合FISH和遗传分析，我们将候选区域缩小到40 kb–1.5 Mb，这与先前基于蝴蝶Melitaea cinxia基因组的先前估计非常吻合。未观察到功能基因的候选区域长度的显着差异可能反映了融合事件后的进化时间。

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