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Genome Size Evolution in Pufferfish: A Comparative Analysis of Diodontid and Tetraodontid Pufferfish Genomes

机译:河豚的基因组大小演变:齿齿和四齿河豚基因组的比较分析。

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摘要

Smooth pufferfish of the family Tetraodontidae have the smallest vertebrate genomes yet measured. They have a haploid genome size of ∼400 million bp (Mb), which is almost eight times smaller than the human genome. Given that spiny pufferfish from the sister family Diodontidae and a fish from the outgroup Molidae have genomes twice as large as smooth puffers, it appears that the genome size of smooth puffers has contracted in the last 50–70 million years since their divergence from the spiny puffers. Here we use renaturation kinetics to compare the repetitive nature of the smooth and spiny puffer genomes. We also estimate the rates of small (<400 bp) insertions and deletions in smooth and spiny puffers using defunct non-LTR retrotransposons. We find a significantly greater abundance of a transposon-like repetitive DNA class in spiny puffers relative to smooth puffers, in addition to nearly identical indel rates. We comment on the role that large insertions may play in the evolution of genome size in these two groups.[The sequence data from this study have been submitted to GenBank under accession nos. AY212336–AY212504.]
机译:四齿科的光滑河豚具有迄今测得的最小的脊椎动物基因组。它们的单倍体基因组大小约为4亿bp(Mb),几乎是人类基因组的八倍。考虑到姐妹家族狄奥多科(Diodontidae)的多刺河豚和野鸭科(Molidae)的一条鱼类的基因组是平滑河豚的两倍,因此看来,自从它们离开多刺以来,最近的50-7000万年间,平滑河豚的基因组大小已经缩小了。膨化。在这里,我们使用复性动力学来比较光滑和多刺河豚基因组的重复性质。我们还估计使用无效的非LTR逆转座子在光滑和多刺的膨化器中小的(<400 bp)插入和缺失的速率。我们发现,与光滑的河豚相比,多刺的河豚中转座子样重复DNA类的丰度要高得多,此外插入缺失率几乎相同。我们评论了大插入可能在这两组基因组大小的进化中所起的作用。[这项研究的序列数据已以登录号提交给GenBank。 AY212336–AY212504。]

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