首页> 美国卫生研究院文献>Genetics >A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and Comparative Genomics in Ambystoma
【2h】

A Comprehensive Expressed Sequence Tag Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and Comparative Genomics in Ambystoma

机译:老虎Sal和墨西哥A的完整的表达序列标签链接图:在Ambystoma中启用基因定位和比较基因组学。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and for A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map for these model amphibians. We identified 14 large linkage groups (125.5–836.7 cM) that presumably correspond to the 14 haploid chromosomes in the Ambystoma genome. The extent of genome coverage for these linkage groups is apparently high because the total map size (5251 cM) falls within the range of theoretical estimates and is consistent with independent empirical estimates. Unlike most vertebrate species, linkage map size in Ambystoma is not strongly correlated with chromosome arm number. Presumably, the large physical genome size (∼30 Gbp) is a major determinant of map size in Ambystoma. To demonstrate the utility of this resource, we mapped the position of two historically significant A. mexicanum mutants, white and melanoid, and also met, a quantitative trait locus (QTL) that contributes to variation in metamorphic timing. This new collection of EST-based PCR markers will better enable the Ambystoma system by facilitating development of new molecular probes, and the linkage map will allow comparative studies of this important vertebrate group.
机译:表达的序列标签(EST)标记被开发用于虎斑小花虾(Eastn Tiger salamander)和墨西哥曲霉(墨西哥。),以生成这些模型两栖动物的第一个全面的连锁图谱。我们鉴定出14个大连锁群(125.5–836.7 cM),它们大概对应于Ambystoma基因组中的14个单倍体染色体。这些连接基团的基因组覆盖范围显然很高,因为总图谱大小(5251 cM)落在理论估计值的范围内,并且与独立的经验估计值一致。与大多数脊椎动物不同,Ambystoma中连锁图谱的大小与染色体臂数没有密切关系。据推测,较大的物理基因组大小(约30 Gbp)是Ambystoma中图谱大小的主要决定因素。为了证明该资源的实用性,我们绘制了两个具有历史意义的墨西哥曲霉突变体白色和黑色素的位置,并且还遇到了导致性状变迁的定量性状基因座(QTL)。基于EST的PCR标记的这一新集合将通过促进新分子探针的开发来更好地启用Ambystoma系统,并且连锁图将允许对该重要脊椎动物群进行比较研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号