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Genome Analysis of a Novel Broad Host Range Proteobacteria Phage Isolated from a Bioreactor Treating Industrial Wastewater

机译:从生物反应器处理工业废水中分离的新型新型广泛宿主蛋白菌噬菌体的基因组分析。

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摘要

Bacteriophages are viruses that infect bacteria, and consequently they have a major impact on the development of a microbial population. In this study, the genome of a novel broad host range bacteriophage, Aquamicrobium phage P14, isolated from a wastewater treatment plant, was analyzed. The Aquamicrobium phage P14 was found to infect members of different Proteobacteria classes (Alphaproteobacteria and Betaproteobacteria). This phage contains a 40,551 bp long genome and 60% of its genes had blastx hits. Furthermore, the bacteriophage was found to share more than 50% of its genes with several podoviruses and has the same gene order as other polyvalent bacteriophages. The results obtained in this study led to the conclusion that indeed general features of the genome of the Aquamicrobium phage P14 are shared with other broad host range bacteriophages, however further analysis of the genome is needed in order to identify the specific mechanisms which enable the bacteriophage to infect both Alphaproteobacteria and Betaproteobacteria.
机译:噬菌体是感染细菌的病毒,因此它们对微生物种群的发展具有重大影响。在这项研究中,分析了从废水处理厂分离出的新型广泛宿主噬菌体Aquamicrobium噬菌体P14的基因组。发现Aquamicrobium噬菌体P14感染不同变形杆菌种类(α变形细菌和Beta变形细菌)的成员。该噬菌体包含一个40,551 bp长的基因组,其60%的基因受到了blastx的攻击。此外,发现该噬菌体与几种足病毒共享其基因的50%以上,并且具有与其他多价噬菌体相同的基因顺序。这项研究获得的结果得出结论:Aquamicrobium噬菌体P14基因组的一般特征确实与其他广泛宿主范围的噬菌体共有,但是需要对基因组进行进一步分析,以鉴定使噬菌体成为可能的特定机制。同时感染Alphaproteobacteria和Betaproteobacteria。

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