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首页> 美国卫生研究院文献>Genes >Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach
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Analysis of DNA Methylation Patterns Associated with In Vitro Propagated Globe Artichoke Plants Using an EpiRADseq-Based Approach

机译：基于EpiRADseq的方法分析与朝鲜蓟朝鲜菊繁殖相关的DNA甲基化模式

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Globe artichoke represents one of the main horticultural species of the Mediterranean basin, and ‘Spinoso sardo’ is the most widespread and economically relevant varietal type in Sardinia, Italy. In the last decades, in vitro culture of meristematic apices has increased the frequency of aberrant plants in open-field production. These off-type phenotypes showed highly pinnate-parted leaves and late inflorescence budding, and emerged from some branches of the true-to-type ‘Spinoso sardo’ plants. This phenomenon cannot be foreseen and is reversible through generations, suggesting the occurrence of epigenetic alterations. Here, we report an exploratory study on DNA methylation patterns in off-type/true-to-type globe artichoke plants, using a modified EpiRADseq technology, which allowed the identification of 2897 differentially methylated loci (DML): 1998 in CG, 458 in CHH, and 441 in CHG methylation contexts of which 720, 88, and 152, respectively, were in coding regions. Most of them appeared involved in primary metabolic processes, mostly linked to photosynthesis, regulation of flower development, and regulation of reproductive processes, coherently with the observed phenotype. Differences in the methylation status of some candidate genes were integrated with transcriptional analysis to test whether these two regulation levels might interplay in the emergence and spread of the ‘Spinoso sardo’ non-conventional phenotype.
机译：朝鲜蓟代表了地中海盆地的主要园艺物种之一，“ Spinoso sardo”是意大利撒丁岛最广泛且与经济相关的品种。在过去的几十年中，分生性尖顶的体外培养增加了露天生产中异常植物的发生频率。这些异型表型显示出高度羽状分开的叶子和晚花序出芽，并从真实型“ Spinoso sardo”植物的某些分支中出现。这种现象是无法预见的，并且是世代可逆的，表明表观遗传改变的发生。在这里，我们报告了使用改良的EpiRADseq技术对变型/真型朝鲜蓟植物中的DNA甲基化模式进行的探索性研究，该技术可识别CG中的2897个差异甲基化基因座（DML）：1998年，458个。 CHH和441在CHG甲基化环境中，其中720、88和152分别位于编码区。它们中的大多数似乎参与了主要的代谢过程，主要与光合作用，花发育的调节和生殖过程的调节有关，与观察到的表型一致。将某些候选基因甲基化状态的差异与转录分析相结合，以测试这两个调节水平是否可能在“ Spinoso sardo”非常规表型的出现和扩散中相互作用。

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