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The Arabidopsis HOBBIT gene encodes a CDC27 homolog that links the plant cell cycle to progression of cell differentiation

机译:拟南芥HOBBIT基因编码CDC27同源物该同源物将植物细胞周期与细胞分化进程联系起来

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摘要

In plant meristems, dividing cells interpret positional information and translate it into patterned cell differentiation. Here we report the molecular identification of the Arabidopsis HOBBIT gene that is required for cell division and cell differentiation in meristems. We show that it encodes a homolog of the CDC27 subunit of the anaphase-promoting complex (APC). HOBBIT partially complements a yeast nuc2/cdc27 mutant. Unlike other CDC27 homologs in Arabidopsis, its transcription is cell cycle regulated. Furthermore, hobbit mutants show a reduction in DR5 :: GUS auxin reporter gene expression and accumulate the AXR3/IAA17 repressor of auxin responses. HOBBIT activity may thus couple cell division to cell differentiation by regulating cell cycle progression in the meristem or by restricting the response to differentiation cues, such as auxin, to dividing cells.
机译:在植物分生组织中,分裂细胞解释位置信息并将其转化为模式化的细胞分化。在这里,我们报告分生组织的细胞分裂和细胞分化所需的拟南芥HOBBIT基因的分子鉴定。我们表明,它编码后期促进复合物(APC)CDC27亚基的同源物。 HOBBIT部分补充酵母nuc2 / cdc27突变体。与拟南芥中的其他CDC27同源物不同,其转录受细胞周期调控。此外,霍比特人突变体显示DR5 :: GUS生长素报告基因基因表达减少,并积累了生长素应答的AXR3 / IAA17阻遏物。因此,HOBBIT活性可以通过调节分生组织中的细胞周期进程或通过限制对分化提示(例如生长素)对分裂细胞的响应来将细胞分裂与细胞分化联系起来。

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