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Natural Variation at sympathy for the ligule Controls Penetrance of the Semidominant Liguleless narrow-R Mutation in Zea mays

机译:触须的同情自然变异控制玉米中半显性无齿无窄R突变的穿透性

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摘要

Leaf architecture determines plant structural integrity, light harvesting, and economic considerations such as plant density. Ligules, junctions at the leaf sheath and blade in grasses, protect stalks from environmental stresses and, in conjunction with auricles, controls leaf angle. Previous studies in mutants have recessive liguleless mutants (lg1 and lg2) and dominant mutations in knotted1-like homeobox genes (Lg3-O, Lg4, and Kn1) involved in ligule development. Recently, a new semidominant liguleless mutant, Liguleless narrow (Lgn-R), has been characterized in maize that affects ligule and auricle development and results in a narrow leaf phenotype. We show that quantitative genetic variation affects penetrance of Lgn-R. To examine the genetic architecture underlying Lgn-R expressivity, crosses between Lgn-R/+ mutants in a B73 background and intermated B73 x Mo17 recombinant inbred lines were evaluated in multiple years and locations. A single main-effect quantitative trait locus (QTL) on chromosome 1 (sympathy for the ligule; sol) was discovered with a Mo17-contributed allele that suppressed Lgn-R mutant phenotypes. This QTL has a genetic-interaction with a locus on chromosome 7 (lucifer; lcf) for which the B73-contributed allele increases the ability of the solMo17 allele to suppress Lgn-R. Neither of the genetic intervals likely to contain sol or lcf overlap with any current liguleless genes nor with previously identified genome-wide association QTL connected to leaf architecture. Analysis of phenotypes across environments further identified a genotype by enviroment interaction determining the strength of the sol x lcf interaction.
机译:叶子的结构决定了植物的结构完整性,光的收集以及经济方面的考虑因素,例如植物的密度。在草中叶鞘和叶片的交界处的利古耳保护茎免受环境压力,并与耳廓一起控制叶的角度。以前在突变体中的研究有隐性无舌的突变体(lg1和lg2)和参与舌齿发育的类似knotted1的同源盒基因(Lg3-O,Lg4和Kn1)中的显性突变。最近,一种新的半显性无齿突变体,无齿窄变(Lgn-R),已经在玉米中得到了表征,影响了舌叶和耳廓的发育并导致了窄叶表型。我们证明了定量遗传变异会影响Lgn-R的渗透率。为了检查Lgn-R表达基础的遗传结构,在多年和地点评估了B73背景中的Lgn-R / +突变体与已确定的B73 x Mo17重组自交系之间的杂交。发现在第1号染色体上有一个单一的主要效应数量性状基因座(QTL)(对叶舌同情; sol),其Mo17贡献的等位基因抑制了Lgn-R突变表型。该QTL与7号染色体上的一个基因位点有相互作用(荧光素; lcf),B73贡献的等位基因为其增加了 sol Mo17 等位基因抑制< em> Lgn-R 。可能包含 sol lcf 的遗传间隔都不会与任何当前的无舌基基因重叠,也不会与先前确定的与叶结构相连的全基因组关联QTL重叠。跨环境表型的分析进一步通过环境相互作用确定了 sol x lcf 相互作用强度的基因型。

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