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首页> 美国卫生研究院文献>G3: GenesGenomesGenetics >Genome Assembly and Annotation of the Medicinal Plant Calotropis gigantea a Producer of Anticancer and Antimalarial Cardenolides
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Genome Assembly and Annotation of the Medicinal Plant Calotropis gigantea a Producer of Anticancer and Antimalarial Cardenolides

机译：药用植物Calotropis gigantea的基因组组装和注释抗癌和抗疟疾胡萝卜素的产生者

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摘要

Calotropis gigantea produces specialized secondary metabolites known as cardenolides, which have anticancer and antimalarial properties. Although transcriptomic studies have been conducted in other cardenolide-producing species, no nuclear genome assembly for an Asterid cardenolide-producing species has been reported to date. A high-quality de novo assembly was generated for C. gigantea, representing 157,284,427 bp with an N50 scaffold size of 805,959 bp, for which quality assessments indicated a near complete representation of the genic space. Transcriptome data in the form of RNA-sequencing libraries from a developmental tissue series was generated to aid the annotation and construction of a gene expression atlas. Using an ab initio and evidence-driven gene annotation pipeline, 18,197 high-confidence genes were annotated. Homologous and syntenic relationships between C. gigantea and other species within the Apocynaceae family confirmed previously identified evolutionary relationships, and suggest the emergence or loss of the specialized cardenolide metabolites after the divergence of the Apocynaceae subfamilies. The C. gigantea genome assembly, annotation, and RNA-sequencing data provide a novel resource to study the cardenolide biosynthesis pathway, especially for understanding the evolutionary origin of cardenolides and the engineering of cardenolide production in heterologous organisms for existing and novel pharmaceutical applications.
机译：Calotropis gigantea产生专门的次生代谢产物，称为心得醇，具有抗癌和抗疟疾特性。尽管已在其他产烯醇内酯的物种中进行了转录组学研究，但迄今为止，尚未报道用于生产紫杉醇烯醇内酯的物种的核基因组装配。为巨型假丝酵母产生了高质量的从头组装，代表157,284,427 bp，N50支架大小为805,959 bp，其质量评估表明基因空间几乎完全代表。生成了来自发育组织系列的RNA测序文库形式的转录组数据，以帮助注释和构建基因表达图谱。使用从头算和证据驱动的基因注释管道，对18,197个高可信度基因进行了注释。巨大假单胞菌和夹竹桃科中其他物种之间的同源和同系关系证实了先前确定的进化关系，并暗示了夹竹桃科亚家族分化后专门的烯醇内酯代谢物的出现或丢失。 C. gigantea基因组装配，注释和RNA测序数据提供了一种新颖的资源来研究芥子内酯的生物合成途径，特别是对于了解芥子内酯的进化起源和异源生物中芥子内酯的生产工程，以用于现有和新型药物应用。

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