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Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles

机译:通过光学显微镜和半导体纳米粒子对生物分子进行纳米级测量

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摘要

Over the past decade, great developments in optical microscopy have made this technology increasingly compatible with biological studies. Fluorescence microscopy has especially contributed to investigating the dynamic behaviors of live specimens and can now resolve objects with nanometer precision and resolution due to super-resolution imaging. Additionally, single particle tracking provides information on the dynamics of individual proteins at the nanometer scale both in vitro and in cells. Complementing advances in microscopy technologies has been the development of fluorescent probes. The quantum dot, a semi-conductor fluorescent nanoparticle, is particularly suitable for single particle tracking and super-resolution imaging. This article overviews the principles of single particle tracking and super resolution along with describing their application to the nanometer measurement/observation of biological systems when combined with quantum dot technologies.
机译:在过去的十年中,光学显微镜的巨大发展使该技术与生物学研究日益兼容。荧光显微镜特别有助于研究活体标本的动态行为,并且由于超分辨率成像,现在可以以纳米精度和分辨率分辨物体。此外,单粒子跟踪可提供有关体外和细胞内纳米级单个蛋白质动力学的信息。显微镜技术的补充进步是荧光探针的发展。量子点是一种半导体荧光纳米粒子,特别适用于单粒子跟踪和超分辨率成像。本文概述了单粒子跟踪和超分辨率的原理,并描述了它们在与量子点技术结合使用时在纳米测量/观测生物系统中的应用。

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