首页> 美国卫生研究院文献>Frontiers in Neuroanatomy >Putative Adult Neurogenesis in Old World Parrots: The Congo African Grey Parrot (Psittacus erithacus) and Timneh Grey Parrot (Psittacus timneh)
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Putative Adult Neurogenesis in Old World Parrots: The Congo African Grey Parrot (Psittacus erithacus) and Timneh Grey Parrot (Psittacus timneh)

机译:在旧世界鹦鹉中推测的成年神经发生:刚果非洲灰鹦鹉(Psittacus erithacus)和Timneh灰鹦鹉(Psittacus timneh)

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摘要

In the current study, we examined for the first time, the potential for adult neurogenesis throughout the brain of the Congo African grey parrot (Psittacus erithacus) and Timneh grey parrot (Psittacus timneh) using immunohistochemistry for the endogenous markers proliferating cell nuclear antigen (PCNA), which labels proliferating cells, and doublecortin (DCX), which stains immature and migrating neurons. A similar distribution of PCNA and DCX immunoreactivity was found throughout the brain of the Congo African grey and Timneh grey parrots, but minor differences were also observed. In both species of parrots, PCNA and DCX immunoreactivity was observed in the olfactory bulbs, subventricular zone of the lateral wall of the lateral ventricle, telencephalic subdivisions of the pallium and subpallium, diencephalon, mesencephalon and the rhombencephalon. The olfactory bulb and telencephalic subdivisions exhibited a higher density of both PCNA and DCX immunoreactive cells than any other brain region. DCX immunoreactive staining was stronger in the telencephalon than in the subtelencephalic structures. There was evidence of proliferative hot spots in the dorsal and ventral poles of the lateral ventricle in the Congo African grey parrots at rostral levels, whereas only the dorsal accumulation of proliferating cells was observed in the Timneh grey parrot. In most pallial regions the density of PCNA and DCX stained cells increased from rostral to caudal levels with the densest staining in the nidopallium caudolaterale (NCL). The widespread distribution of PCNA and DCX in the brains of both parrot species suggest the importance of adult neurogenesis and neuronal plasticity during learning and adaptation to external environmental variations.
机译:在本研究中,我们首次使用免疫组化技术检测增殖细胞核抗原(PCNA)的免疫组化方法,研究了刚果非洲灰鹦鹉(Psittacus erithacus)和蒂姆氏灰鹦鹉(Psittacus timneh)整个大脑的成年神经发生潜能。 )(可标记增殖细胞)和双皮质素(DCX)(可染色未成熟和正在迁移的神经元)。在整个刚果非洲灰鹦鹉和Timneh灰鹦鹉的大脑中发现了PCNA和DCX免疫反应性的相似分布,但也观察到了微小差异。在这两种鹦鹉中,在嗅球,侧脑室侧壁的脑室下区域,大脑皮层和大脑皮层的脑下细分,中脑,中脑和横脑中均观察到PCNA和DCX免疫反应。嗅球和远脑区的PCNA和DCX免疫反应细胞密度均高于其他任何大脑区域。 DCX免疫反应性染色在端脑中比在脑下亚结构中强。有证据表明,在非洲非洲灰鹦鹉的鼻侧水平处,其侧脑室的背侧和腹侧极处有增生性热点,而在Timneh灰鹦鹉中仅观察到了增殖细胞的背侧积聚。在大多数丘脑区域,PCNA和DCX染色的细胞密度从延髓到尾部水平增加,其中刺鼻侧柏(NCL)的染色最密集。两种鹦鹉物种的大脑中PCNA和DCX的广泛分布表明在学习和适应外部环境变化过程中,成年神经发生和神经元可塑性的重要性。

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