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首页> 美国卫生研究院文献>Frontiers in Neuroanatomy >Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)
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Analysis of shape and spatial interaction of synaptic vesicles using data from focused ion beam scanning electron microscopy (FIB-SEM)

机译：使用聚焦离子束扫描电子显微镜（FIB-SEM）的数据分析突触小泡的形状和空间相互作用

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The spatial interactions of synaptic vesicles in synapses were assessed after a detailed characterization of size, shape, and orientation of the synaptic vesicles. We hypothesized that shape and orientation of the synaptic vesicles are influenced by their movement toward the active zone causing deviations from spherical shape and systematic trends in their orientation. We studied three-dimensional representations of synapses obtained by manual annotation of focused ion beam scanning electron microscopy (FIB-SEM) images of male mouse brain. The configurations of synaptic vesicles were regarded as marked point patterns, where the points are the centers of the vesicles, and the mark of a vesicle is given by its size, shape, and orientation characteristics. Statistics for marked point processes were employed to study spatial interactions between vesicles. We found that the synaptic vesicles in excitatory synapses appeared to be of oblate ellipsoid shape and in inhibitory synapses appeared to be of cigar ellipsoid shape, and followed a systematic pattern regarding their orientation toward the active zone. Moreover, there was strong evidence of spatial alignment in the orientations of pairs of synaptic vesicles, and of repulsion between them only in excitatory synapses, beyond that caused by their physical extent.
机译：在详细描述突触小泡的大小，形状和方向后，评估突触小泡在突触中的空间相互作用。我们假设突触小泡的形状和方向受其向活动区移动的影响，从而导致其偏离球形和系统趋势。我们研究了通过手动标注男性小鼠大脑聚焦离子束扫描电子显微镜（FIB-SEM）图像获得的突触的三维表示。突触小泡的构型被认为是标记的点模式，其中的点是小泡的中心，小泡的标记由其大小，形状和方向特征给出。使用标记点过程的统计数据来研究囊泡之间的空间相互作用。我们发现，兴奋性突触中的突触小泡似乎呈扁球形，而抑制性突触中的突触小泡则呈雪茄椭球形，并且它们朝着活性区的方向遵循系统的模式。此外，有强有力的证据表明，成对的突触小泡在空间取向上是对齐的，并且仅在兴奋性突触中它们之间的排斥作用超出了它们的物理范围。

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