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首页> 美国卫生研究院文献>Frontiers in Microbiology >Impact of Hurricane Irene on Vibrio vulnificus and Vibrio parahaemolyticus concentrations in surface water sediment and cultured oysters in the Chesapeake Bay MD USA
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Impact of Hurricane Irene on Vibrio vulnificus and Vibrio parahaemolyticus concentrations in surface water sediment and cultured oysters in the Chesapeake Bay MD USA

机译：艾琳飓风对美国马里兰州切萨皮克湾地表水沉积物和牡蛎中创伤弧菌和副溶血弧菌浓度的影响

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To determine if a storm event (i.e., high winds, large volumes of precipitation) could alter concentrations of Vibrio vulnificus and V. parahaemolyticus in aquacultured oysters (Crassostrea virginica) and associated surface water and sediment, this study followed a sampling timeline before and after Hurricane Irene impacted the Chesapeake Bay estuary in late August 2011. Aquacultured oysters were sampled from two levels in the water column: surface (0.3 m) and near-bottom (just above the sediment). Concentrations of each Vibrio spp. and associated virulence genes were measured in oysters with a combination of real-time PCR and most probable number (MPN) enrichment methods, and in sediment and surface water with real-time PCR. While concentration shifts of each Vibrio species were apparent post-storm, statistical tests indicated no significant change in concentration for either Vibrio species by location (surface or near bottom oysters) or date sampled (oyster tissue, surface water, and sediment concentrations). V. vulnificus in oyster tissue was correlated with total suspended solids (r = 0.41, P = 0.04), and V. vulnificus in sediment was correlated with secchi depth (r = -0.93, P <0.01), salinity (r = -0.46, P = 0.02), tidal height (r = -0.45, P = 0.03), and surface water V. vulnificus (r = 0.98, P <0.01). V. parahaemolyticus in oyster tissue did not correlate with environmental measurements, but V. parahaemolyticus in sediment and surface water correlated with several measurements including secchi depth [r = -0.48, P = 0.02 (sediment); r = -0.97, P <0.01 (surface water)] and tidal height [r = -0.96, P <0.01 (sediment), r = -0.59, P <0.01 (surface water)]. The concentrations of Vibrio spp. were higher in oysters relative to other studies (average V. vulnificus 4 × 105 MPN g-1, V. parahaemolyticus 1 × 105 MPN g-1), and virulence-associated genes were detected in most oyster samples. This study provides a first estimate of storm-related Vibrio density changes in oyster tissues, sediment, and surface water at an aquaculture facility in the Chesapeake Bay.
机译：为了确定风暴事件（即强风，大量降水）是否会改变水产养殖牡蛎（Crassostrea virginica）及相关地表水和沉积物中的创伤弧菌和副溶血弧菌的浓度，本研究遵循了采样前后的时间线2011年8月下旬，艾琳飓风袭击了切萨皮克湾河口。从水柱中的两个水位采样了水产养殖牡蛎：地表水（0.3 m）和近水底牡蛎（正好在沉积物上方）。每个弧菌的浓度。通过实时PCR和最可能数（MPN）富集方法对牡蛎中的相关毒力基因和相关毒力基因进行测定，并通过实时PCR在牡蛎中的沉积物和地表水中进行测定。尽管风暴后每种弧菌的浓度变化明显，但统计测试表明，按地点（表层牡蛎或近地牡蛎）或取样日期（牡蛎组织，地表水和沉积物的浓度），弧菌的浓度没有明显变化。牡蛎组织中的创伤弧菌与总悬浮固体相关（r = 0.41，P = 0.04），沉积物中的创伤弧菌与secchi深度（r = -0.93，P <0.01），盐度（r = -0.46）相关，P = 0.02），潮高（r = -0.45，P = 0.03），和地表水 V。创伤（ r = 0.98， P <0.01）。 V。牡蛎组织中的副溶血与环境测量值无关，但与 V相关。沉积物和地表水中的副溶血菌与多种测量相关，包括secchi深度[ r = -0.48， P = 0.02（沉积）； r = -0.97， P <0.01（地表水）]和潮高[ r = -0.96， P <0.01（沉积物）， r = -0.59， P <0.01（地表水）]。 弧菌 spp的浓度。牡蛎相对于其他研究而言更高（平均 V。vulnificus 4×10 5 MPN g -1 ，副溶血弧菌< / em> 1×10 5 MPN g -1 ），并且在大多数牡蛎样品中检测到了毒力相关基因。这项研究提供了切萨皮克湾水产养殖设施中牡蛎组织，沉积物和地表水与风暴有关的弧菌密度变化的初步估计。

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