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Structural insights into diversity and n-alkane biodegradation mechanisms of alkane hydroxylases

机译:烷烃羟化酶多样性和正构烷烃生物降解机制的结构见解

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摘要

Environmental microbes utilize four degradation pathways for the oxidation of n-alkanes. Although the enzymes degrading n-alkanes in different microbes may vary, enzymes functioning in the first step in the aerobic degradation of alkanes all belong to the alkane hydroxylases. Alkane hydroxylases are a class of enzymes that insert oxygen atoms derived from molecular oxygen into different sites of the alkane terminus (or termini) depending on the type of enzymes. In this review, we summarize the different types of alkane hydroxylases, their degrading steps, and compare typical enzymes from various classes with regard to their three-dimensional structures, in order to provide insights into how the enzymes mediate their different roles in the degradation of n-alkanes and what determines their different substrate ranges. Through the above analyzes, the degrading mechanisms of enzymes can be elucidated and molecular biological methods can be utilized to expand their catalytic roles in the petrochemical industry or in bioremediation of oil-contaminated environments.
机译:环境微生物利用四个降解途径来氧化正构烷烃。尽管在不同微生物中降解正构烷烃的酶可能会有所不同,但在有氧降解烷烃的第一步中起作用的酶均属于烷烃羟化酶。烷烃羟化酶是一类酶,根据酶的类型,它们可将源自分子氧的氧原子插入烷烃末端(或末端)的不同位置。在这篇综述中,我们总结了不同类型的烷烃羟化酶,它们的降解步骤,并就其三维结构比较了各种类别的典型酶,以便深入了解这些酶如何介导它们在降解降解过程中的不同作用。正构烷烃以及决定其不同底物范围的因素。通过以上分析,可以阐明酶的降解机理,并可以利用分子生物学方法扩大其在石油化学工业或石油污染环境的生物修复中的催化作用。

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