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首页> 美国卫生研究院文献>Frontiers in Genetics >Development of SSR Markers and Assessment of Genetic Diversity in Medicinal Chrysanthemum morifolium Cultivars
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Development of SSR Markers and Assessment of Genetic Diversity in Medicinal Chrysanthemum morifolium Cultivars

机译：药用菊花栽培品种SSR标记的开发及遗传多样性评估

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摘要

Chrysanthemum morifolium, is a well-known flowering plant worldwide, and has a high commercial, floricultural, and medicinal value. In this study, simple-sequence repeat (SSR) markers were generated from EST datasets and were applied to assess the genetic diversity among 32 cultivars. A total of 218 in silico SSR loci were identified from 7300 C. morifolium ESTs retrieved from GenBank. Of all SSR loci, 61.47% of them (134) were hexa-nucleotide repeats, followed by tri-nucleotide repeats (17.89%), di-nucleotide repeats (12.39%), tetra-nucleotide repeats (4.13%), and penta-nucleotide repeats (4.13%). In this study, 17 novel EST-SSR markers were verified. Along with 38 SSR markers reported previously, 55 C. morifolium SSR markers were selected for further genetic diversity analysis. PCR amplification of these EST-SSRs produced 1319 fragments, 1306 of which showed polymorphism. The average polymorphism information content of the SSR primer pairs was 0.972 (0.938–0.993), which showed high genetic diversity among C. morifolium cultivars. Based on SSR markers, 32 C. morifolium cultivars were separated into two main groups by partitioning of the clusters using the unweighted pair group method with arithmetic mean dendrogram, which was further supported by a principal coordinate analysis plot. Phylogenetic relationship among C. morifolium cultivars as revealed by SSR markers was highly consistent with the classification of medicinal C. morifolium populations according to their origin and ecological distribution. Our results demonstrated that SSR markers were highly reproducible and informative, and could be used to evaluate genetic diversity and relationships among medicinal C. morifolium cultivars.
机译：菊花是世界著名的开花植物，具有很高的商业，花卉和医学价值。在这项研究中，从EST数据集生成了简单序列重复（SSR）标记，并将其用于评估32个品种之间的遗传多样性。从GenBank检索到的7300个Morifolium EST中总共鉴定出218个计算机SSR基因座。在所有SSR基因座中，其中61.47％（134）是六核苷酸重复序列，其次是三核苷酸重复序列（17.89％），二核苷酸重复序列（12.39％），四核苷酸重复序列（4.13％）和五核苷酸核苷酸重复序列（4.13％）。在这项研究中，验证了17个新的EST-SSR标记。连同先前报道的38个SSR标记一起，选择了55个C. morifolium SSR标记用于进一步的遗传多样性分析。这些EST-SSR的PCR扩增产生1319个片段，其中1306个片段显示出多态性。 SSR引物对的平均多态性信息含量为0.972（0.938-0.993），这表明mo旋栽培种之间具有很高的遗传多样性。基于SSR标记，使用具有算术平均树状图的非加权对组方法，通过群集的划分，将32个mo旋栽培种分为两个主要组，这进一步得到了主坐标分析图的支持。通过SSR标记揭示的mo兰栽培种之间的亲缘关系与根据其起源和生态分布对药用C兰种群的分类高度一致。我们的结果表明，SSR标记具有很高的重现性和信息性，可用于评估药用假单胞菌栽培品种之间的遗传多样性和相关性。

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