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首页> 美国卫生研究院文献>Frontiers in Bioengineering and Biotechnology >Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication
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Ultra-Low-Cost 3D Bioprinting: Modification and Application of an Off-the-Shelf Desktop 3D-Printer for Biofabrication

机译：超低成本3D生物打印：用于生物制造的现成台式3D打印机的修改和应用

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摘要

3D bioprinting has become a versatile and powerful method in tissue engineering and regenerative medicine and is increasingly adapted by other disciplines due to its tremendous potential beyond its typical applications. However, commercially available 3D bioprinting systems are typically expensive circumventing the broad implementation, including laboratories in low-resource settings. To address the limitations of conventional and commercially available technology, we developed a 3D bioprinter by modification of an off-the-shelf 3D desktop printer, that can be installed within a single day, is of handy size to fit into a standard laminar flow hood, customizable, ultra-low cost and thus, affordable to a broad range of research labs, or educational institutions. We evaluate accuracy and reproducibility of printing results using alginate and alginate/gelatin-hydrogels and demonstrate its potential for biomedical use by printing of various two-and three-dimensional cell-free and mammalian cell-laden objects using recombinant HEKYFP cells, stably expressing yellow fluorescent protein (YFP) as a model system and high-content imaging. We further provide a parts list and 3D design files in STL and STEP format for reconstructing the device. A time-lapse video of the custom-built device during operation is available at .
机译：3D生物打印已成为组织工程和再生医学中一种通用且功能强大的方法，并且由于其超越常规应用的巨大潜力，越来越多地被其他学科所采用。但是，商业上可买到的3D生物打印系统通常价格昂贵，无法实现广泛的应用，包括资源匮乏的实验室。为了解决传统技术和商用技术的局限性，我们通过修改现成的3D台式打印机来开发3D生物打印机，该打印机可以在一天之内安装，尺寸方便，可装入标准层流罩中，可定制，超低成本，因此可为各种研究实验室或教育机构买得起。我们使用藻酸盐和藻酸盐/明胶水凝胶评估印刷结果的准确性和可重复性，并通过使用重组HEK YFP <印刷各种二维和三维无细胞和哺乳动物细胞的物体来证明其在生物医学中的潜力/ sup>细胞，可稳定表达黄色荧光蛋白（YFP）作为模型系统和高内涵成像。我们还提供了STL和STEP格式的零件清单和3D设计文件，用于重建设备。定制设备在运行过程中的延时视频可在访问。

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