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首页> 美国卫生研究院文献>Frontiers in Bioengineering and Biotechnology >Bioconversion of Raw Glycerol From Waste Cooking-Oil-Based Biodiesel Production to 13-Propanediol and Lactate by a Microbial Consortium
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Bioconversion of Raw Glycerol From Waste Cooking-Oil-Based Biodiesel Production to 13-Propanediol and Lactate by a Microbial Consortium

机译：微生物财团将原始甘油从以烹饪用油为基础的生物柴油生产中生物转化为13-丙二醇和乳酸

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摘要

Waste cooking oil (WCO) is a sustainable alternative to raw vegetable oils and fats for biodiesel production considering both environmental and economic benefits. Raw glycerol from WCO-based biodiesel production (GWCO) is difficult to utilize via biological method, as multiple toxic impurities have inhibitory effects on microbial growth especially for pure cultures. In this work, four microbial consortia were selected from activated sludge by 30 serial transfers under different conditions. The obtained consortia exhibited lower diversity and species difference with the transfers. The consortium LS30 exhibited unique advantages for bioconversion of GWCO to 1,3-propanediol (1,3-PDO) and lactate (LA). Moreover, the fermentation could be performed economically under microaerobic and non-sterile conditions. The consortium consisted of 57.97% Enterobacter and 39.25% Escherichia could effectively convert 60 g/L GWCO to 1,3-PDO and LA in batch fermentation. In addition, this consortium exhibited better tolerance to fatty acid-derived crude glycerol (100 g/L), which demonstrated that specific toxic impurities in GWCO did pose a great challenge to microbial growth and metabolism. In fed batch fermentation, 27.77 g/L 1,3-PDO and 14.68 g/L LA were achieved. Compared with the consortium, a long lag phase in cell growth associated with a decreased glycerol consumption was observed in four single-strain fermentations. Furthermore, neither the consortium DL38 with excellent glycerol tolerance nor consortium C2-2M with high yield of 1,3-PDO could effectively transform GWCO into valuable products. The results demonstrated that the selected microbial consortium has the advanced adaptability to the toxic impurities in GWCO compared with other reported consortia and isolated single strain. This process can contribute to added-value use of GWCO.
机译：考虑到环境和经济利益，废食用油（WCO）是生物柴油生产中植物油和油脂的可持续替代品。基于WCO的生物柴油生产（GWCO）中的粗甘油很难通过生物方法加以利用，因为多种有毒杂质对微生物的生长具有抑制作用，尤其是对于纯培养物。在这项工作中，通过在不同条件下进行30次连续转移从活性污泥中选择了四个微生物联盟。获得的财团表现出较低的多样性和物种差异。 LS30联盟展示了将GWCO生物转化为1,3-丙二醇（1,3-PDO）和乳酸（LA）的独特优势。而且，可以在微需氧和非无菌条件下经济地进行发酵。该联盟由57.97％的肠杆菌和39.25％的大肠杆菌组成，可以在分批发酵中有效地将60 g / L GWCO转化为1,3-PDO和LA。此外，该财团对源自脂肪酸的粗甘油（100 g / L）表现出更好的耐受性，这表明GWCO中特定的有毒杂质确实对微生物的生长和代谢提出了巨大挑战。在分批补料发酵中，获得了27.77 g / L的1,3-PDO和14.68 g / L的LA。与财团相比，在四个单菌株发酵中观察到细胞生长的长期滞后阶段与甘油消耗的减少有关。此外，具有出色的甘油耐受性的财团DL38和具有高产率的1,3-PDO的财团C2-2M都不能有效地将GWCO转化为有价值的产品。结果表明，与其他报道的财团和分离出的单一菌株相比，所选择的微生物财团对GWCO中的有毒杂质具有更高的适应性。这个过程可以促进GWCO的增值使用。

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