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Isolation and Identification of Acholeplasma sp. from the Mud Crab Scylla serrata

机译:破壁孢子虫的分离与鉴定来自泥蟹Scyla serrata

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摘要

For the first time, a mollicute-like organism (MLO) was cultured from moribund mud crabs (Scylla serrata) during an outbreak of clearwater disease in Zhejiang Province, China. The MLO displayed a fried-egg colony morphology in culture, did not possess a cell wall, and was not retained by 0.45 μm and 0.2 μm filters. It was able to ferment glucose, sucrose, lactose, and maltose, but it did not utilize arginine and urea. The MLO grew in the absence of bovine serum and was not susceptible to digitonin. Sequence analysis of the 16S rRNA gene revealed that this MLO had 99% identity with Acholeplasma laidlawii PG-8A, which indicates that the organism isolated from mud crabs is a member of the genus Acholeplasma.
机译:在中国浙江省爆发的清水病期间,第一次从垂死的泥蟹(Scylla serrata)中培养出了类软体动物(MLO)。 MLO在培养中表现出煎蛋菌落形态,不具有细胞壁,并且没有被0.45μm和0.2μm的过滤器保留。它能够发酵葡萄糖,蔗糖,乳糖和麦芽糖,但没有利用精氨酸和尿素。 MLO在没有牛血清的情况下生长,并且不易受洋地黄皂苷的影响。对16S rRNA基因的序列分析表明,该MLO与无毛破壁虫PG-8A具有99%的同一性,这表明从泥蟹中分离出的生物体是无泡破壁菌的成员。

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