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首页> 美国卫生研究院文献>Evidence-based Complementary and Alternative Medicine : eCAM >The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats
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The Predicted Proteomic Network Associated with the Antiarthritic Action of Qingfu Guanjieshu in Collagen-II-Induced Arthritis in Rats

机译：预测的蛋白质组网与庆福冠捷舒在II型胶原诱导的关节炎中的抗关节炎作用

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Qingfu Guanjieshu (QFGJS) is an herbal preparation for treating rheumatoid arthritis (RA). Previous studies revealed that QFGJS significantly inhibited experimental arthritis and acute inflammation, accompanied by reduction of proinflammatory cytokines and elevation of anti-inflammatory cytokines. This study aims to identify the targeted proteins and predict the proteomic network associated with the drug action of QFGJS by using 2D gel and MALDI-TOF-MS/MS techniques. Thirty female Wistar rats were evenly grouped as normal and vehicle- and QFGJS-treated CIA rats. The antiarthritic effect of QFGJS was examined with a 19-day treatment course, and the knee synovial tissues of animals from each group were obtained for 2D gel and MALDI-TOF-MS/MS analysis. Results showed that QFGJS significantly ameliorated collagen II-induced arthritis when administrated at 2.8 g/kg body weight for 19 days. 2D gel image analysis revealed 89 differentially expressed proteins in the synovial tissues among the normal and vehicle- and QFGJS-treated CIA rats from over 1000 proteins of which 63 proteins were identified by MALDI-TOF-MS/MS analysis, and 32 proteins were included for classification of functions using Gene Ontology (GO) method. Finally, 14 proteins were analyzed using bioinformatics, and a predicted proteomic network related to the anti-arthritic effect of QFGJS was established, and Pgk1 plays a central role.
机译：清肤关解术（QFGJS）是一种用于治疗类风湿关节炎（RA）的草药。先前的研究表明，QFGJS可显着抑制实验性关节炎和急性炎症，并伴有促炎细胞因子减少和抗炎细胞因子升高。这项研究旨在通过使用2D凝胶和MALDI-TOF-MS / MS技术鉴定目标蛋白并预测与QFGJS的药物作用相关的蛋白质组网络。将三十只Wistar雌性大鼠平均分为正常大鼠，经媒介物和QFGJS治疗的CIA大鼠。用19天的疗程检查QFGJS的抗关节炎作用，并从每组动物的膝滑膜组织中获得2D凝胶和MALDI-TOF-MS / MS分析。结果表明，当以2.8μg/ kg体重施用19天时，QFGJS显着改善了II型胶原引起的关节炎。二维凝胶图像分析显示，正常和媒介物以及QFGJS处理的CIA大鼠的滑膜组织中89种差异表达的蛋白质，其中1000多种蛋白质通过MALDI-TOF-MS / MS分析鉴定出63种蛋白质，其中包括32种蛋白质使用基因本体（GO）方法进行功能分类。最后，使用生物信息学分析了14种蛋白质，并建立了与QFGJS抗关节炎作用相关的预测蛋白质组网络，Pgk1发挥了核心作用。

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