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首页> 美国卫生研究院文献>Evidence-based Complementary and Alternative Medicine : eCAM >Phyllanthus Suppresses Prostate Cancer Cell PC-3 Proliferation and Induces Apoptosis through Multiple Signalling Pathways (MAPKs PI3K/Akt NFκB and Hypoxia)
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Phyllanthus Suppresses Prostate Cancer Cell PC-3 Proliferation and Induces Apoptosis through Multiple Signalling Pathways (MAPKs PI3K/Akt NFκB and Hypoxia)

机译：竹兰抑制前列腺癌细胞PC-3增殖并通过多种信号通路（MAPKPI3K / AktNFκB和低氧）诱导凋亡。

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Phyllanthus is a traditional medicinal plant that has been found to have antihepatitis, antibacterial, and anticancer properties. The present studies were to investigate the in vitro molecular mechanisms of anticancer effects of Phyllanthus (P. amarus, P. niruri, P. urinaria, and P. watsonii) plant extracts in human prostate adenocarcinoma. The cancer ten-pathway reporter array was performed and revealed that the expression of six pathway reporters were significantly decreased (Wnt, NFκB, Myc/Max, hypoxia, MAPK/ERK, and MAPK/JNK) in PC-3 cells after treatment with Phyllanthus extracts. Western blot was conducted and identified several signalling molecules that were affected in the signalling pathways including pan-Ras, c-Raf, RSK, Elk1, c-Jun, JNK1/2, p38 MAPK, c-myc, DSH, β-catenin, Akt, HIF-1α, GSK3β, NFκB p50 and p52, Bcl-2, Bax, and VEGF, in treated PC-3 cells. A proteomics-based approach, 2D gel electrophoresis, was performed, and mass spectrometry (MS/MS) results revealed that there were 72 differentially expressed proteins identified in treated PC-3 cells and were involved in tumour cell adhesion, apoptosis, glycogenesis and glycolysis, metastasis, angiogenesis, and protein synthesis and energy metabolism. Overall, these findings suggest that Phyllanthus can interfere with multiple signalling cascades involved in tumorigenesis and be used as a potential therapeutic candidate for treatment of cancer.
机译：余甘子是一种传统的药用植物，具有抗肝炎，抗菌和抗癌的特性。本研究旨在研究叶兰（P. amarus，P。niruri，P。urinaria和P. watsonii）植物提取物在人前列腺腺癌中的抗癌作用的体外分子机制。进行了癌症十通路报告基因阵列的分析，结果显示，在用竹节鱼处理后，PC-3细胞中六种通路报告基因的表达显着降低（Wnt，NFκB，Myc / Max，缺氧，MAPK / ERK和MAPK / JNK）。提取物。进行了Western印迹分析并鉴定了在信号通路中受影响的几种信号分子，包括pan-Ras，c-Raf，RSK，Elk1，c-Jun，JNK1 / 2，p38 MAPK，c-myc，DSH，β-catenin，处理过的PC-3细胞中的Akt，HIF-1α，GSK3β，NFκBp50和p52，Bcl-2，Bax和VEGF。进行了基于蛋白质组学的方法2D凝胶电泳，质谱（MS / MS）结果显示，在经过处理的PC-3细胞中鉴定出72种差异表达的蛋白质，并参与肿瘤细胞的粘附，凋亡，糖原生成和糖酵解，转移，血管生成以及蛋白质合成和能量代谢。总体而言，这些发现表明，叶楠可以干扰参与肿瘤发生的多个信号级联反应，并且可以用作治疗癌症的潜在候选药物。

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