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首页> 美国卫生研究院文献>Evidence-based Complementary and Alternative Medicine : eCAM >Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components
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Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components

机译：超高效液相色谱/混合四极杆-Orbitrap质谱分析和初步筛选抗流感病毒成分的四芒雀麦化学成分的定性和定量分析

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Tetrastigma hemsleyanum Diels et Gilg (T. hemsleyanums) is a kind of traditional folk medicinal plant which has been used widely in China for its antivirus, antitumor, and other clinical effects. In this study, ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap mass spectrometry (UPLC-Q-Exactive/MS) was utilized to analyze the chemical constituents of T. hemsleyanums. Fifty-one constituents were clarified, including flavonoids, anthraquinones, esters, fatty acids, phenols, and catechins. In the subsequent quantitative analysis, the contents of ten compounds of rutin, kaempferol, astragalin, quercitrin, quercetin, vitexin-rhamnoside, isorhamnetin, vitexin, emodin-8-O-β-D-glucoside, and isoquercetin in 18 batches of T. hemsleyanums collected from different places of cultivation were determined. Meanwhile, anti-influenza virus bioactivity in vitro of the above samples was detected with Gaussia Luciferase viral titer assay. It was found that the antiviral bioactivity varied from batches to batches in accordance with content difference of the chemical constituents in T. hemsleyanums. Correlation analysis was performed with SPSS software for the association between LC-MS chemometrics and bioactivity of influenza virus inhibition, and 8 constituents of flavonoids showed positive correlation coefficient, which may provide a valuable clue for searching potential antiviral components in T. hemsleyanums.
机译：四角四叶草（T. hemsleyanums）是一种传统的民间药用植物，由于其抗病毒，抗肿瘤和其他临床作用已在中国得到广泛应用。在这项研究中，超高效液相色谱结合混合四极杆-轨道质谱仪（UPLC-Q-Exactive / MS）被用于分析棉铃虫的化学成分。澄清了五十一种成分，包括类黄酮，蒽醌，酯，脂肪酸，酚和儿茶素。在随后的定量分析中，对18批T中的十种芦丁，山tin酚，黄芪素，槲皮素，槲皮素，黄体素-鼠李糖苷，异鼠李素，黄体素，大黄素-8-O-β-D-葡萄糖苷和异槲皮素的含量进行了分析。测定了从不同栽培场所收集的半边芹。同时，采用高斯荧光素酶病毒滴度法检测了上述样品的体外抗流感病毒的生物活性。已经发现，根据棉铃虫中化学成分的含量差异，抗病毒生物活性随批次而变化。用SPSS软件进行相关分析，以分析LC-MS化学计量学与抑制流感病毒的生物活性之间的关系，并且8个类黄酮成分显示出正相关系数，这可能为寻找棉铃虫中潜在的抗病毒成分提供了有价值的线索。

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