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首页> 美国卫生研究院文献>European Journal of Histochemistry : EJH >Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats
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Effect of thyroxine on munc-18 and syntaxin-1 expression in dorsal hippocampus of adult-onset hypothyroid rats

机译：甲状腺素对成年甲状腺功能减退大鼠背侧海马munc-18和syntaxin-1表达的影响

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Adult-onset hypothyroidism induces a variety of impairments on hippocampus- dependent neurocognitive functioningin which many synaptic proteins in hippocampus neurons are involved. Here, we observed the effect of adult-onset hypothyroidism on the expression of syntaxin-1 and munc-18 in the dorsal hippocampus and whether the altered proteins could be restored by levothyroxine (T4) treatment. All rats were separated into 4 groups randomly: hypothyroid group, 5 µg T4 /100 g body weight (BW) treated group, 20 µg T4/100 g BW treated group and control group. The radioimmunoassay kits were applied to assay the levels of serum T3 and T4, and the levels of syntaxin-1 and munc-18 in hippocampus were assessed by immunohistochemistry and Western blot. Both analysis corroborated that syntaxin-1 in the hypothyroid group was significantly higher. Munc-18 was lower in four layers of CA3 and dentate gyrus by immunohistochemistry. After two weeks of treatment with 5 µg T4/100 g BW for hypothyroidism, syntaxin-1 levels were completely restored, whereas the recovery of munc-18 only located in two of the four impaired layers. Twenty µg T4/100 g BW treatment normalized munc-18 levels. These data suggested that adult-onset hypothyroidism induced increment of syntaxin-1 and decrement of munc-18 in the dorsal hippocampus, which could be restored by T4 treatment. Larger dosage of T4 caused more effective restorations.
机译：成年甲状腺功能减退症会引起海马依赖性神经认知功能的各种损伤，其中涉及海马神经元中的许多突触蛋白。在这里，我们观察了成年甲状腺功能减退症对背海马中syntaxin-1和munc-18表达的影响，以及是否可以通过左甲状腺素（T4）治疗恢复被改变的蛋白质。将所有大鼠随机分为4组：甲状腺功能减退组，5 µg T4 / 100 g体重（BW）治疗组，20 µg T4 / 100 g BW治疗组和对照组。应用放射免疫分析试剂盒检测血清T3和T4水平，并通过免疫组织化学和Western blot检测海马中syntaxin-1和munc-18的水平。两项分析均证实甲状腺功能减退组中的syntaxin-1明显更高。通过免疫组织化学，在CA3和齿状回的四层中，Munc-18较低。用5 µg T4 / 100 g BW治疗甲状腺功能减退症两周后，syntaxin-1水平完全恢复，而munc-18的恢复仅位于四个受损层中的两个。 20 µg T4 / 100 g BW处理将munc-18水平标准化。这些数据表明，成年甲状腺功能减退症可引起背海马的syntaxin-1升高和munc-18降低，这可以通过T4治疗得以恢复。 T4的剂量越大，修复效果越好。

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